摘要
本文采用RACE技术,从巴西橡胶树中克隆到一个小热激蛋白基因。该基因全长cDNA为1 002 bp,最长开放阅读框645 bp,预测编码蛋白包含214个氨基酸残基,与植物sHSP23.8蛋白具高度相似,将该基因命名为HbsHSP23.8。生物信息学分析显示,HbsHSP23.8具有α-晶体蛋白保守结构域,可能定位在叶绿体,HbsHSP23.8蛋白中预测到19个磷酸化位点。二级结构预测结果显示HbsHSP23.8由α螺旋、β转角、延伸链和随机卷曲组成。实时定量RT-PCR分析结果表明,HbsHSP23.8在巴西橡胶树胶乳、叶片、树皮、雄花、雌花、花药中均有表达。在橡胶树叶片不同发育时期,HbsHSP23.8表达存在明显变化。此外,HbsHSP23.8表达受高盐、干旱、低温、乙烯和茉莉酸处理调控,表明HbsHSP23.8可能在巴西橡胶树逆境胁迫应答、乙烯和茉莉酸信号途径中发挥作用。
In this study,a full-length cDNA sequence of a small heat shock protein（sHSP） gene was cloned from Hevea brasiliensis with the RACE method.The full-length cDNA of HbsHSP23.8 was 1 002 bp in size with a 645 bp open reading frame,encoding a deduced polypeptide of 214 amino acids.The deduced protein showed high identity to plant sHSP23.8 proteins,therefore we named this genes as HbsHSP23.8.Bioinformatic analyses indicated that HbsHSP23.8 contained a conserved alpha-crystallin domains（ACD） domain,and was likely located in chloroplast.19 phosphorylation sites were predicted within HbsHSP23.8,and the secondary structure of HbsHSP23.8 contained a-helix,P-turn,extended strand and random coil.Real time RT-PCR analysis indicated that HbsHSP23.8 was expressed in latex,barks,leaves,barks,male flowers,female flowers and anthers.With the development of leaves,the HbsHSP23.8 expression showed a significant change.In addition,the expression of HbsHSP23.8 was regulated by NaCl,drought,low temperature,ET and JA treatments,suggesting that HbsHSP23.8 might play important roles in stresses responses as well as ET and JA signals in rubber tree.
出处
《植物生理学报》
CAS
CSCD
北大核心
2015年第11期1955-1962,共8页
Plant Physiology Journal
基金
国家自然科学基金(31270651和31200514)
中国热带农业科学院橡胶研究所基本科研业务费专项(16300-22015003)
关键词
巴西橡胶树
小热激蛋白23.8
逆境反应
基因克隆
表达分析
Hevea brasiliensis
small heat shock protein 23.8
stress response
gene cloning
expression analysis
作者简介
E-mail：dragonldj@163．com，Tel：0898-66279271
E—mail：zhxia-111@163．com，Tel：0898．23301174；
