摘要
多菌灵是植物病害防治中常用的化学杀菌剂,野生型哈茨木霉对多菌灵比较敏感。为了能够更好地将哈茨木霉菌应用于实践,本试验采用紫外线-氯化锂复合诱变的方法,实现哈茨木霉菌株对多菌灵的抗性改良。试验共获得315株正向突变菌株,其中hcb-35菌株抗性能力较强。利用毒力测定方法检测了多菌灵对hcb-35有效抑菌中浓度,及hcb-35对多菌灵的抗药遗传稳定性;并利用对峙试验及显微观察检测其抑菌能力。结果显示,与哈茨木霉出发菌株hc相比,多菌灵对哈茨木霉突变株hcb-35菌株的有效抑菌中浓度提升285%;连续转接12代后,hcb-35菌株抗药性相对稳定且抑菌效果较出发菌株无明显差异,表明应用紫外-氯化锂复合诱变哈茨木霉可以获得遗传稳定的耐多菌灵突变株。
Carbendazim is one of commonly used chemical fungicides in plant disease control,and wild type Tri-choderma harzianum is very sensitive to carbendazim.In order to make better use of T .harzianum strains,the improvement of carbendazim-resistance in T .harzianum strains was studied by UV-LiCl composite mutagenesis. A total of 31 5 positive mutation strains were obtained,among which the hcb-35 strain was better than others.The 50% effective inhibition concentration （EC50 ）and genetic stability of carbendazim-resistance was detected,and the inhibition efficacy of T .harzianum was tested by the method of confrontation and microscopic observation. The results showed that,compared with the T .harzianum starting strain hc,the EC50 of carbendazim against hcb-35 strain was increased by 285%.The carbendazim-resistance of hcb-35 strain was relatively stable and the inhibi-tion to the target pathogen had no significant difference after subculture for 12 generations.It suggested that the carbendazim-resistant strain of T .harzianum mutated by UV-LiCl could be stably inherited.
出处
《植物保护》
CAS
CSCD
北大核心
2015年第5期79-84,共6页
Plant Protection
基金
国家自然科学基金地区科学基金(21267001)
黑龙江省高校科技创新团队(2012TD006)
研究生创新项目(YJSCX2014-Y60)
关键词
复合诱变
哈茨木霉
多菌灵
抗药性
抑菌效果
composite mutation
Trichoderma harzianum
carbendazim
carbendazim-resistance
inhibition
作者简介
通信作者E-mail：sdmlzw@126．com
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