摘要
该研究选用中国目前应用最多的燃料油刮”柴油为研究对象，阐明不同浓度0^#柴油水溶性成分（diesel oil water—soluble fraction，DWSF）作用下菲律宾蛤仔（Rtwlitapes philippinarum）肝胰腺和鳃组织中几种抗氧化酶活性的响应特征。结果表明，0^#柴油水溶性成分作用下，菲律宾蛤仔过氧化氢酶（CAT）、谷胱甘肽硫转移酶（GST）、超氧化物歧化酶（SOD）与过氧化物酶（POD）活性在暴露的某些时段被显著诱导或抑制，总体而言鳃组织中抗氧化酶活性的响应比肝胰腺敏感。4种抗氧化酶中，CAT、SOD和POD对0^#柴油的响应较为敏感，尤其是鳃组织中POD的活性最高可被显著诱导至对照组的3倍。整合生物标志物（1BR）分析结果表明菲律宾蛤仔抗氧化防御系统对高浓度0^#柴油水溶性成分的响应较为敏感。因此，0^#柴油水溶性成分胁迫会对菲律宾蛤仔体内的抗氧化系统造成损伤，其活性的变化可作为指示海洋石油污染的潜在生物标志。
In this study, we investigated the effect of different concentrations of water-soluble fraction of No. 0 diesel oil （ DWSF）, the most used fuel oil in China, on antioxidant enzymes activities of Manila clam （Ruditapes philippinarum）. The activities of catalase （CAT） , glutathione S-transferase （GST） , superoxide dismutase SOD and peroxidase （POD） were significantly induced or depressed in hepatopancreas and gills of R. philippinarum at different concentrations and time intervals. Generally, the response of antioxidases in gills exhibited higher sensitivity than that in hepatopancreas. Among these four antioxidase, CAT, SOD and POD were highly sensitive to No. 0 diesel oil exposure, especially POD activity in gills with the highest induction of approximate three times of the control. Inte- grated biomarker response （IBR） index reflected that the antioxidant defense systems of R. philippinarum were more sensitive to the high concentration of DWSF. Thus, it is concluded that the water-soluble fraction of No. 0 diesel oil can damage the antioxidant en- zyme system of R. philippinarum, demonstrating great potential as biomarker in biotesting of marine oil pollutions.
出处
《南方水产科学》
CAS
CSCD
北大核心
2015年第6期65-72,共8页
South China Fisheries Science
基金
国家自然科学基金项目(41306113)
农业部渔业局项目(2012-NZ-5739)
中央级公益性科研院所基本科研业务费专项资金(2014A02XK02)
海南省社会发展科技专项资金项目(XH201312)
关键词
菲律宾蛤仔
0^#柴油
水溶性成分
抗氧化防御系统
Ruditapes philippinarum
No. 0 diesel oil
water-soluble fraction
antioxidant enzymes
作者简介
作者简介：张林宝（1984一），女，博士，助理研究员，从事海洋环境毒理学研究。E-mail：zhandinba01984@163．com
通信作者：贾晓平（1949一），男，研究员，从事渔业环境监测与评价研究。E-mail：jxp60@tom．com
