摘要
以新疆大蒜主产区根腐病大蒜根际土壤为研究对象,采用涂布法、最大或然法、Biolog-ECO微孔板等方法分别测定了根际土壤微生物群落组成、生理菌群数量、碳源代谢特征及部分土壤酶活性。结果表明:根腐病大蒜根际土壤中细菌、真菌、放线菌数量都明显高于健康植株,细菌数量在发病后期达到最高,而真菌和放线菌数量呈现先升后降的趋势。感病株根际土壤生理菌群数量明显低于健康株,而蔗糖酶活性是健康株的2倍。感病植株根际土壤微生物代谢水平在发病初期维持较高水平且高于健康株,发病后期急剧下降。多样性指数方面,感病株始终高于健康株。综上所述,感病植株根际土壤微生物群落总体以数量多、多样性丰富而生理代谢活性较低为主要特征。
Taking the rhizosphere soil samples of healthy and root rot diseased garlic plants in Xinjiang area as research objects,dilution separation method, most probable number method, spectrophotometric method and Biolog-ECO plate were adopted to evaluate the microbial community composition of samples, number of physiology group, soil enzyme activities and metabolism characteristics of carbon sources. The results showed that quantities of bacteria ,fungi and aetinomycetes in rhizosphere soil from diseased plants were significantly higher than those from intact plants. In the observation period ,fungi and aetinomyeetes number of effeeted samples showed a rise first followed by a decline. Bacteria number was at record level at the late-stage of root rot disease. Azotobacter, nitrobacter and cellulose decomposing bacteria decreased after infection ,yet at the same time ,the soil invertase activity increased by two times. In the early stage of root rot disease ,carbon sources metabolism of infected rhizosphere soil was higher than healthy rhizosphere soil ,and then dramatic decreased at the late stage. The diversity index of infected samples was higher than healthy ones. In conclusion,large amount microorganism,abundant biodiversity and lower metabolism activity were the major features of the microbial community in rhizosphere soil from diseased garlic plants.
出处
《现代农业科技》
2015年第21期133-135,137,共4页
Modern Agricultural Science and Technology
基金
新疆维吾尔自治区自然科学基金项目(2013211A043)
关键词
根腐病大蒜
根际微生物
群落特征
新疆地区
root rot diseased garlic
rhizosphere microbe
community characteristics
Xinjiang area
