摘要
目的建立职业接触者血铅测定的微分电位溶出法(Differential potentiometric stripping method,DPDD)和氢化物发生原子荧光法(Hydride generation atomic fluorescence method,HGAF),并对两种方法进行比较。方法用DPDD法与HGAF法分别检测同一批职业接触者血清样品以及标准质控样品,以DPDD法为标准方法,以HGAF法为参比法,分析两种检测方法的相关性和相对误差。结果对血清样品的检测,两种方法高度符合(r=0.996,P<0.05),DPDD法的相对标准偏差为2.6%,加标回收率为(94.5±2.8)%;HGAF法相对标准偏差为2.8%,加标回收率为(100.0±6.4)%,两者差异无统计学意义(P>0.05)。两种方法质控样的检测结果均在允许范围内,且差异无统计学意义(P>0.05)。结论 DP-DD法与HGAF法检测血铅均具有较高的准确性,且一致性良好,能够满足血铅检测的要求,但HGAF法仪器价廉,分析速度快,对检测操作要求简单,适合基层单位快速检测的需要。
Objective To set up a determination of blood lead for occupation exposure by differential potentiometricstripping method and hydride generation atomic fluorescence method,and to compare the two methods. Methods The serumsamples and standard quality control samples of the same batch of occupation exposure were detected by differentialpotentiometric stripping method and hydride generation atomic fluorescence spectrometry,differential potentiometric strippingmethod as the standard method,hydride generation atomic fluorescence spectrometry as reference method,the correlation andrelative error of two methods were analyzed. Results Detection with serum samples,two kinds of methods almost the same（r=0.996,P〈0.05）, the analysis error of differential potentiometric stripping was 2.6%, and the analysis error of hydridegeneration atomic fluorescence spectrometric method was 2.8%,addition standard recovery rate were（94.5±2.8）%,（100.0±6.4）%,respectively,there was no significant difference between the two methods（P〈0.05）. Two methods for detecting qualitysamples results were within the allowable range,and there was no significant difference（P〈0.05）. Conclusion Thedifferential potentiometric stripping method and hydride generation atomic fluorescence spectrometric method have highaccuracy and good consistency,and can meet the requirements of blood lead detection. The hydride generation atomicfluorescence method instrument is cheap with fast speed of analysis, and the require of detection operation is simple,which issuitable for the rapid detection of the grass-roots units.
出处
《中国热带医学》
CAS
2015年第10期1278-1280,共3页
China Tropical Medicine
关键词
微分电位溶出法
氢化物发生原子荧光法
血铅
职业接触者
Differential potentiometric stripping method
Hydride generation atomic fluorescence spectrometry method
Blood lead
Occupation exposure
作者简介
叶裕正（1975-），男，大专，主管技师，研究方向：卫生检验学。
