摘要
目的探讨丹参酮ⅡA对重症急性胰腺炎(severe acute pancreatitis,SAP)肺损伤(lung iniury,LI)大鼠的改善作用及其可能机制。方法予SD大鼠注射牛磺胆酸钠建立SAP模型(简称SAP组),并在此基础上给予丹参酮ⅡA(sodium tanshinoneⅡA sulfonate,STS)干预(简称STS组),同时设立假手术对照组(简称SO组),每组24只大鼠。观察各组大鼠的生存状态、肺脏湿干重比、MPO试剂盒测定肺组织髓过氧化物酶(MPO)活性、Hofbuaer方法测定肺组织病理损伤、采用E LISA方法测定肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(lL-1β)、采用免疫组化方法测定细胞间黏附分子-1(ICAM-1)表达水平。结果 SAP组大鼠生存状态恶化,肺脏湿干重比、肺组织MPO活性、肺组织病理损伤评分以及细胞因子TNF-α、IL-1β和ICAM-1均明显高于SO组,而与SAP组比较,STS组大鼠生存状态好转,肺脏湿干重比、肺组织MPO活性、肺组织病理损伤评分及TNF-α、IL-1β、ICAM-1水平均明显下降,差异均有统计学意义(P<0.05)。结论丹参酮ⅡA对大鼠SAPLI有明显改善作用,其机制可能与改变肺脏细胞因子水平、减轻肺脏炎性细胞浸润有关。
Objective To explore the effect of Tanshinone ⅡA on severe acute pancreatitis（SAP） lung injury（ALI） rats and its possible mechanism.Methods SD rats were injected with sodium taurocholate to induce SAP group,and then intervened with sodium tanshinone ⅡA sulfonate（STS group）.Simultaneously a sham-operation group（SO group） was set up.There were 24 rats in each group.The survival state and wet-to-dry weight ratio of lung tissues were observed.Activities of myeloperoxidase（MPO） in lung were determined by MPO reagent kit.Pathologic changes of lung tissues were determined by Hofbuaer method.Expression levels of three cytokines,TNF-α,IL-1β,and intercellular cell adhesion molecule-1（ICAM-1） were detected by ELISA.Results The survival state of rats in the SAP group was deteriorated.The wet-to-dry weight ratio,MPO activities,pathologic changes in lung tissues,and expression levels of TNF-α,IL-1β,and ICAM-1 increased significantly more in the SAP group than in the SO group（P 0.05）.Compared with those in the SAP group,the survival state of rats in the STS group was improved;the wet-to-dry weight ratio,MPO activities,pathologic changes in lung tissues,and expression levels of TNF-α,IL-1β,and ICAM-1 obviously decreased in the STS group（P 0.05）.Conclusion Tanshinone ⅡA had remarkable effect on SPA LI rats,which might be associated with changing cytokines levels and attenuating infiltration of lung inflammatory cells.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2015年第11期1361-1366,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
南京市医学科技发展项目(No.YKK11086)
作者简介
通讯作者：刘明东，Tel：025-83106666转61319，E-mail：njglyy@163．com
