摘要
目的:观察针康法对脑缺血大鼠脑组织血管内皮生长因子(VEGF)、层粘连蛋白(LN)表达的影响,探讨针康法促进神经功能恢复的相关作用机制。方法:建立大鼠永久性局灶性脑缺血模型,设立模型组、假手术组、针刺组、康复组及针康组。模型组、假手术组不予任何治疗,针刺组采用头穴丛刺针法治疗,康复组采用跑台训练模拟临床康复治疗,针康组采用针康法治疗。免疫组织化学法观察各时间点缺血脑组织VEGF、LN的动态变化。结果:术后7天、14天,与模型组相比,康复组、针刺组与针康组VEGF阳性表达增多(P<0.05);与康复组、针刺组比较,针康组VEGF阳性细胞数明显增多(P<0.05)。术后7天、14天,与模型组相比,康复组、针刺组与针康组LN阳性表达增多(P<0.05);与康复组、针刺组比较,针康组LN阳性细胞数明显增多(P<0.05)。结论:针康法可能通过上调缺血脑组织VEGF、LN的表达促进脑缺血后神经功能恢复。
Objective： To observe the expression of VEGF and LN in brain tissue of cerebral ischemia rats by acupuncture- rehabilitation,and to explore the mechanism of acupuncture- rehabilitation to promote the recovery of nerve function. Methods： The rat models of permanent focal cerebral ischemia were established. They were divided into model group,sham operation group,acupuncture group,rehabilitation group and acupuncture- rehabilitation group. Model group and sham operation group were without any treatment; the acupuncture group was treated by scalp acupuncture treatment; the rehabilitation group received treadmill training simulating clinical rehabilitation; acupuncture group was with acupuncture combined with rehabilitation. The changes of VEGF and LN in ischemic brain tissue at different time points were observed by immunohistochemistry. Results： After7 d,14 d compared with the model group and rehabilitation group,in the acupuncture group and acupuncture-rehabilitation group the expression of VEGF was increased（ P〈0. 05）; compared with the rehabilitation group and the acupuncture group,in the acupuncture- rehabiliation group the number of VEGF positive cells was increased significantly（ P〈0. 05）. After 7 d,14 d compared with the model group,rehabilitation group,acupuncture group and acupuncture- rehabilitation group the expression of LN was increased（ P〈0. 05）; compared with the rehabilitation group and acupuncture group,in the acupuncture- rehabilitation group the number of LN positive cells was increased significantly（ P〈0. 05）. Conclusion： Acupuncture- rehabilitation promotes the recovery of neural function after cerebral ischemia by increasing the expression of VEGF and LN in ischemic brain tissue.
出处
《针灸临床杂志》
2015年第10期71-74,共4页
Journal of Clinical Acupuncture and Moxibustion
基金
黑龙江省教育厅科学技术研究项目
编号:12531606
黑龙江中医药大学科研基金项目
编号:201202
作者简介
朱路文（1983-），男，主治医师，主要从事脑缺血针灸康复研究。
通讯作者：唐强（1963-），男，教授，博士研究生导师，主要从事神经系统疾病的中医康复研究。
