摘要
以十六烷基三甲基溴化铵 (CTAB)为阳离子表面活性剂 ,用胶束电动毛细管色谱 (MECC)分别对抑肽酶粉针剂和抑肽酶注射液进行纯度测定。实验中选择了最佳缓冲液 (含 4mmol/LCTAB的 80mmol/LNa2 HPO4 H3 PO4,pH 7 0 0 ) ,考察了进样量与样品中高浓度盐对分离的影响。并对毛细管区带电泳、MECC和高效液相色谱的分离效果加以比较 ,表明MECC的分离效果最佳。
A sensitive and rapid method was developed for purity determination of different types of aprotinin by micellar electrokinetic capillary chromatography (MECC). MECC was performed with 80 mmol/L Na 2HPO 4 H 3PO 4 (pH 7 00) buffer solution containing 4 mmol/L cetyltrimethyl ammonium bromide (CTAB) at an applied voltage of 15 kV. The electrophoresis was monitored at 277 nm, and completed in 6 minutes. The relative standard deviations of migration time and area for aprotinin were below 0 3% and below 5% respectively within 1 day (for 6 repetitive runs ). The effect of high concentration of salt in the samples has been studied. The results obtained by capillary zone electrophoresis, MECC and high performance liquid chromatography were compared, and those of MECC were the best.
出处
《色谱》
CAS
CSCD
北大核心
2002年第4期353-355,共3页
Chinese Journal of Chromatography
关键词
胶束电动毛细管色谱
抑肽酶
粉针剂
注射液
纯度测定
生物药品
micellar electrokinetic capillary chromatography
aprotinin
powder for injection
injection
purity determination
