摘要
为研究浙江省稻瘟病菌群体的遗传多样性,利用分布于稻瘟病菌7条染色体上的28对SSR引物,对浙江省11个城市1978至2011年11个城市的稻瘟病菌的遗传结构多样性进行了分析。结果表明,28对SSR引物共扩增出171个等位基因,每对引物可扩出2～24个等位基因,平均多样性指数0.534,平均多态性信息含量为0.494。分离于不同地理位置的稻瘟病菌株的多样性较高,其中丽水和杭州的遗传多样性最高,舟山的遗传多样性最低。分离于不同年份的稻瘟病菌的遗传结构差异较大,1990年的遗传多样最高,1978年的遗传多样性最低。分离于不同水稻类型的稻瘟病菌的遗传多样性较高,部分分离于籼稻和粳稻的稻瘟病菌的遗传结构相似甚至相同,分离于籼稻的稻瘟病菌的遗传多样性高于分离于粳稻的稻瘟病菌。
Twenty-eight pairs of SSR primers that distributed on 7 chromosomes of Magnaporthe oryzae were used to study the genetic diversity of M. oryzae isolates from Zhejiang Province. M. oryzae strains isolated from 11 different areas in Zhejiang Province,from 1978 to 2011 were used as materials. Results showed that the total amplified alleles were 171,the amplified alleles of each primer pairs was 2 to 24,the average diversity index was 0. 534 and the average polymorphism information content was 0. 494. The genetic structure of M. oryzae strains isolated from different areas was different,Lishui and Hangzhou populations had the highest genetic diversity and Zhoushan population had the lowest genetic diversity. Genetic diversities of M. oryzae isolates from different years were different,the 1990 population had the highest genetic diversity,while the 1978 population had the lowest genetic diversity. The genetic structure of M. oryzae isolated from different rice types was different,a part of strains isolated from indica and japonica had the similarity genetic structure,and the strains isolated from indica rice had the higher genetic diversity than those isolated from japonica rice.
出处
《浙江农业学报》
CSCD
北大核心
2015年第10期1781-1788,共8页
Acta Agriculturae Zhejiangensis
基金
浙江省公益技术研究农业项目(2013C32019,2012C22024)
浙江省自然科学基金(Y3110537)
农业部公益性行业项目(201203014)
关键词
稻瘟病菌
SSR
遗传结构
多样性
Magnaporthe oryzae
SSR
genetic structure
diversity
作者简介
谢晶晶（1989-），男，浙江杭州人，硕士研究生，研究方向为植物病理。E-mail：455422906@qq．com
通讯作者，孙国昌，E-mail：sungc01@sina．com
