摘要
Aim To investigate the nephroprotective activity of berberine in diabetic nephropathy （DN） mice. Methods Diabetic nephropathy was induced by intraperitoneal injection with 55 mg · kg^-1 streptozotocin（STZ） , Berberine was administered at daily doses of 50, 100 and 200 mg· kg^-1 by gavage for 8 weeks. To detect serum creatinine and blood urea nitrogen （BUN） levels, blood were collected after the last dose of berberine, renal cortex was separated on ice after heart peffusion by precooled normal saline. The specimen was stored in -80℃ for the next experiments, and some of the kidney tissue were immobilized by 4% paraformaldehyde solution and 3% glut- araldehyde solution for the preparation of paraffin tissue slides and electron microscope biopsy respectively. After that, PAS staining and electron microscope were used to observe the glomerular morphology changes; ELISA was used to measure proinflammatory chemokines and cytokines levels in renal cortex. Real time RT-PCR was taken to detect the level of nucleotide binding oligomerzation domain 2 （NOD2） mRNA, Western blot was used to test the ex- pression of NOD2 and autophagy marker light chain 3 （LC3） in renal cortex. Results Histopathological changes and the increase in serum creatinine and BUN in DN mice were significantly ameliorated by berberine in a dose-de- pendent manner. Additionally, The expression of tumor necrosis factor-or （TNF-α）, interleukin-6 （IL-6） and in- tercellular adhesion molecule-1 （ICAM-1） was markedly suppressed by berberine, indicating the inhibition of in- flammatory response. Treatment of DN mice with berberine also significantly reduced the expression of NOD2 and LC3 in the kidneys. Conclusion The current study showed the nephroprotective activity of berberine in DN mice could be attributed to the inhibition of inflammation and
出处
《中国药理学通报》
CAS
CSCD
北大核心
2015年第B11期97-97,共1页
Chinese Pharmacological Bulletin
