摘要
利用浮游植物荧光仪对暴露于不同浓度白玉兰落叶水浸出液下微囊藻生长、最大光合作用效率(Fv/Fm)、实际光合作用效率[Y(Ⅱ)]、光能利用效率(alpha)和最大相对电子传递速率(r ETRmax)进行为期15 d的检测,分析白玉兰落叶浸出液对微囊藻的抑制效应和叶绿素荧光特性影响.结果发现,白玉兰落叶浸出液能有效抑制微囊藻的生长,呈明显浓度抑制型变化,抑藻能力随时间的延长而下降.低浓度(0.4、0.8、1.2、1.6 g·L-1)浸出液胁迫下,对微囊藻叶绿素荧光参数无显著影响;高浓度(2.0 g·L-1)浸出液胁迫下,在早期(4 d内)对荧光参数有极显著抑制作用.三维荧光图谱表明,在投量为2.0 g·L-1时,第15天色氨酸及酪氨酸荧光峰强度约为1.2 g·L-1投量情况下的1/3,同时腐殖酸的荧光峰强度减弱.第7—15天,藻细胞生长的半抑制浓度EC50值最小约为0.5—0.7 g·L-1.
The growth and physiology of bloom-forming M. aeruginosa were determined by the pulse amplitude modulated fluorimetry when exposed to aqueous extract of M. denudata defoliation for15 d. The cell growth,maximal efficiency（ Fv/ Fm）,effective quantum yield（ Y（ Ⅱ）） of PSⅡphotochemistry,photosynthesis efficiency（ alpha） and maximum relative electron transport rate（ r ETRmax） were used to evaluate the growth and photosynthesis in M. aeruginosa. The extract stored for 5 days significantly inhibited the growth of M. aeruginosa in a concentration-dependent way. It showed that low concentrations（ 0. 4,0. 8,1. 2 and 1. 6 g·L^-1） of the extract had little effect on the four chlorophyll fluorescence parameters. However,high concentration（ 2. 0 g·L^-1） of the extract significantly affected the fluorescence parameters at early stage（ 4 d）. Three-dimensional fluorescence spectroscopy showed that on day 15 of the treatment the peak intensity of tryptophan and tyrosine fluorescence with 2. 0 g·L^-1extract is about a third of than 1. 2 g·L^-1extract. At the same time,the peak intensity of humic acid fluorescence was much weaker.On 7—15 d,the EC50 of algal growth was the smallest which was about 0. 5—0. 7 g·L^-1.
出处
《环境化学》
CAS
CSCD
北大核心
2015年第10期1867-1874,共8页
Environmental Chemistry
基金
国家自然科学基金(51378316)
公益性行业水利科研专项(201301047)
深圳市科技局(JCYJ20130331151242230
JCYJ201504301657307)资助
关键词
白玉兰
铜绿微囊藻
化感作用
浮游植物荧光仪
Magnolia denudate
Microcystis aeruginosa
allelopathy
pulse amplitude modulated fluorimetry
作者简介
通讯联系人：E—mail：wangxiaoxiong20@szpt．edu．cn
