摘要
目的：测定龙芍续骨胶囊中芍药苷的含量,并考察中性氧化铝层析纯化对芍药苷含量测定的影响。方法：采用高效液相色谱法测定芍药苷含量,以中性氧化铝纯化富集芍药苷。液相条件：Kromasil-C18（250mm×4.6 mm,5μm）色谱柱;以甲醇-水（28∶72）为流动相,流速为1.0 m L·min-1,检测波长为230 nm,柱温30℃。结果：芍药苷在0.048~1.68μg范围内与峰面积呈良好线性关系（r=0.9996）,精密度和稳定性试验的RSD分别为0.31%和0.86%,平均回收率为99.19%,RSD为0.97%（n=6）。结论：本方法简单,稳定易行,灵敏度高,可控性强,重复性好,可用于龙芍续骨胶囊中芍药苷的含量测定。建议在本品质量标准中规定每1 g内容物含白芍以芍药苷（C23H28O11）计,不得少于0.38 mg。
Objective：To establish a method for determination of paeoniflorin in Longshao Xugucapsule and study on the determination of neutral alumina chromatography purification effect of paeoniflorin. Method：Determination of paeoniflorin content by HPLC,purification and concentration of paeoniflorin in neutral alumina. The HPLC method was carried out on Cromasil C18（150 mm×4.6 mm,5 μm）column using Methanol-water（28∶72）as mobile phase,the flow rate was 1.0 m L·min-1,The detection wavelength was 230 nm,detection wavelength was 230 nm.The tempterature of column was 30℃. Result：Paeoniflorin in 0.048~1.68 μg range showed a good linear relationship（r=0.9996）;Precision and stability test of RSD were 0.31% and 0.86%,the average recovery rate of（n=6）99.19%,RSD 0.97%. Conclusion：The method is simple,stable and easy operation,high sensitivity,strong controllability,repeatability,and can be used for content determination of paeoniflorin in long shao xu gu capsule. Recommendations are provided for every 1 g content of paeoniflorin in Radix Paeoniae Alba by containing the quality standard of this product（C23H28O11）meter,shall not be less than 0.38 mg.
出处
《辽宁中医药大学学报》
CAS
2015年第10期51-53,共3页
Journal of Liaoning University of Traditional Chinese Medicine
作者简介
王道东（1978-），男，山东聊城人，主管药师，学士，研究方向：药剂学。
