摘要
目的探讨黄芩茎叶总黄酮(SSTF)对大鼠缺血再灌注大脑皮质神经元突触超微结构的保护作用。方法雄性SD大鼠20只,随机分为假手术组、模型组、SSTF组[SSTF 100mg/(kg·d)]和尼莫地平组[尼莫地平1mg/(kg·d)],每组5只。造模前1周给药,后3组建立大脑中动脉局灶性脑缺血再灌注模型,Longa法行神经功能缺损评分,电镜观察大脑皮质缺血区神经元和突触的超微结构变化。结果模型组大鼠神经功能缺损评分(2.81±0.96)分,神经元和突触超微结构破坏;与模型组比较,SSTF组神经功能缺损评分降低,为(1.48±0.98)分,P<0.05,神经元和突触超微结构较明显好转,大部分突触超微结构趋于正常,尼莫地平组变化与SSTF组接近(P>0.05)。结论 SSTF可通过减轻大脑皮质神经元和突触超微结构损伤,保护缺血再灌注脑神经功能。
Objective To study the role of scutellaria baicalensis stern-leaf total flavonoid （SSTF） in protecting ultrastructure of cerebral cortex neurons and synapse in rats following I/R injury. Methods Twenty male SD rats were randomly divided into sham operation group,model group, SSTF treatment group and nimodipine （NIM） treatment group （5 in each grou）. Rats in SSTF treatment group and NIM treatment group were treated with SSTF 100 mg/（kg - d） and NIM 1 mg/（kg·d） respectively 1 week before the focal I/R injury model of middle cerebral artery was established. Neurological function was scored according to Longa 5-point scoring system. The ultrastrueture of cerebral cortex neurons and synapse was observed under electron microscope. Results The neurological function score was significantly higher in model group than in SSTF treatment group （2.81±0.96 vs 1.48±0. 98,P（0.05）. The ultrastructure of cerebral cortex neurons and synapses was damaged more significantly in model group than in SSTF treatment group. No significant difference was found in ultrastructure of cerebral cortex neurons and synapses between SSTF treatment group and NIM treatment group. Conclusion SSTF can protect the neurological function in rats following I/R injury by alleviating the damage of ultrastructure of cerebral cortex neurons and synapse.
出处
《中华老年心脑血管病杂志》
CAS
2015年第10期1091-1093,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
河北省科技厅科学技术研究与发展计划(0727610lD46)
关键词
黄芩
类黄酮物质
再灌注
大脑皮质
神经元
突触
scutellaria baicalensis
flavonoids
reperfusion
cerebral cortex
neurons
synapses
作者简介
通信作者：赵淑敏，E-mail：zhaoshumin-2008@163．com
