摘要
目的：探讨影响SysmexXT1800i血液分析仪血小板计数（PLT）准确性的相关因素。方法分别采用电阻抗法、光学法和手工法检测PLT，并探讨平均红细胞体积（MCV）、红细胞碎片（FRC）、血小板参数、大体积幼稚血小板对3种方法检测结果的影响。结果当MCV＜60、60～70fL时3种方法检测PLT结果存在明显差异（P＜0．05）。电阻抗法检测PLT最高（P＜0．05），而手工法和光学法之间PLT结果差异无统计学意义（P＞0．05）。MCV≥70fL且FRC为10％～＜100％和≥100％时电阻抗法PLT高于手工法和光学法（P＜0．05），但手工法和光学法PLT差异无统计学意义（P＞0．05）。当MCV≥70fL且血小板三项参数无法显示时，3种方法PLT差异有统计学意义（P＜0．05），电阻抗法PLT明显高于手工法和光学法（P＜0．05），但手工法和光学法PLT差异无统计学意义（P＞0．05）。大体积幼稚血小板监测1、3、5d，手工法PLT明显高于电阻抗法和光学法，光学法PLT明显高于电阻抗法，差异均有统计学意义（P＜0．01）。结论使用SysmexXT1800i血液分析仪检测PLT时，要根据具体情况采取相应方法。
Objective To investigate the related factors affecting the accuracy of platelet（PLT ） count detected by the Sysmex XT1800i hematologic analyzer .Methods The impedance method ,optical method and manual method were adopted to detect the PLT count .The influence of mean MCV ,FRC ,PLT parameters and large volume imma‐ture PLT on results detected by 3 kinds of method was investigated .Results When MCV 〈 60 ,60 - 70 fL ,the PLT count had statistical difference among 3 kinds of detection method（P〈 0 .01） .The PLT count detected by the imped‐ance method was highest（P 〈 0 .05） ,while the PLT count had no obvious difference between the manual method and the optical method（P〉0 .05） .When MCV ≥ 70 fL and FRC was 10% - 〈 100% and ≥ 100% ,the PLT count detec‐ted by the impedance method was higher than that detected by the manual method and the optical method ,but the PLT count had no significant difference between the manual method and the optical method （ P 〉 0 .05 ） .When MCV ≥ 70 fL and no display of 3 PLT parameters ,the PLT count had statistical difference among 3 kinds of detection method（P〈 0 .05） ,the PLT count detected by the impedance method was significantly higher than that detected by the manual method and the optical method（P 〈 0 .05） ,but the PLT count had no statistical difference between the manual method and the optical method（P〉 0 .05） .In large volume immature PLT monitoring for 1 ,3 ,5 d ,the PLT count detected by the manual method was significantly higher than that detected by the impedance method and the optical method ,the PLT count detected by the optical method was significantly higher than that detected by the im‐pedance method ,the difference was statistically significant（P〉 0 .05） .Conclusion In detecting the PLT counting by using the Sysmex XT1800i hematologic analyzer ,the corresponding detection method should be taken according to the concrete conditions .
出处
《检验医学与临床》
CAS
2015年第19期2842-2844,共3页
Laboratory Medicine and Clinic
基金
重庆市卫生和计划生育委员会基金资助项目(20143065)
关键词
血液分析仪
血小板计数
准确性
光学法
电阻抗法
hematology analyzer
platelet count
accuracy
optical method
resistance method
作者简介
王丹，女，本科，主管检验师，主要从事临床血液学相关研究。
通讯作者，Email：dongyjian@163．com。
