摘要
采用常规的用于测定活细胞个数的试剂2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐(WST-8四唑盐)建立了一种快速测定细胞活性的表面增强拉曼散射光谱(SERS)新方法.考察了p H值及还原剂烟酰胺腺嘌噙二核苷酸(NADH)用量对WST-8甲瓒信号的影响.结果表明,与传统的WST-8比色法相比,SERS法具有更高的检测灵敏度和选择性,对WST-8甲瓒的检测限可达到10μmol/L.
Surface-enhanced Raman scattering（ SERS） technique was used to develop and optimize a novel and quantitative 2-（ 2-methoxy-4-nitrophenyl）-3-（ 4-nitrophenyl）-5-（ 2,4-disulfophenyl）-2H-tetrazolium monosodium salt（ WST-8） assay for living cell viability. The influences of p H and dosage of reductant nicotinamide adenine dinucleotide（ NADH） on WST-8 formazan signal were examined. SERS signals of formazan with high resolution and great diversity between WST-8 and formazan were observed in neutral conditions.Meanwhile,the signals of formazan（ 560,1351,1598,1616 cm-1） could be found clearly when the concentration of NADH was four times that of WST-8. Compared with the traditional method,this assay appears higher sensitivity and selectivity for WST-8 formazan. Moreover,the detection limit of formazan is as low as 10μmol /L.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2015年第10期1900-1905,共6页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:21273091
21221063
21327803
2141114023)
吉林省科技厅青年基金(批准号:20130522181JH)资助~~
作者简介
联系人简介：赵冰，男，博士，教授，博士生导师，主要从事光谱分析研究．E—mail：zhaob@jlu．edu．cn
