摘要
采用四极子（G4）DNA修饰的金纳米棒通过荧光对铅（Ⅱ）进行检测。由于铅（Ⅱ）对四极子DNA结构具有极高的稳定效率,能够驱使四极子DNA形成四极子结构与荧光探针锌原卟啉（ZnPPIX）螯合。金纳米棒不仅作为DNA的载体,而且金纳米棒的荧光增强效应还可以提高ZnPPIX-G4的荧光强度,从而构建了一个灵敏的铅（Ⅱ）荧光传感器。以透射电子显微镜、荧光光谱法及紫外吸收光谱法等表征该四极子DNA-金纳米棒复合材料。该荧光传感器的线性范围为100nmol·L^-1以内和150~1 000nmol·L^-1之间,检出限（3S/N）为0.1nmol·L^-1。采用该检测器对自来水样品进行分析,测定结果与电感耦合等离子体原子发射光谱法的测定结果相符。
Gold nanorods modified by G-quadruplex was used for the fluorescence determination of Pb（Ⅱ）.Pb（Ⅱ）can drive the formation of G-quadruplex DNA which can chelate with the fluorescent probes zinc protoporphyrin because Pb（Ⅱ）can stabilize the G-quadruplex DNA structure with high efficiency.The gold nanorods not only are a DNA carrier,but also enhance the fluorescence intensity of ZnPPIX-G4.Based on the fact,a new Pb（Ⅱ）sensitive fluorescent sensor was constructed. The G-quadruplex DNA-gold nanorods were characterized by transmission electron microscopy,fluorescence spectroscopy and UV absorption spectroscopy.The linearity range of the Pb（Ⅱ）found were within 100nmol·L^-1 and 150-1 000nmol·L^-1,with detection limit（3S/N）of 0.1nmol·L^-1.The sensor was used to analyze tap water samples,giving results in agreement with that obtained by ICP-AES.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2015年第9期1269-1273,共5页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家自然科学基金资助项目(21175156
20975114
20775093)
中国博士后基金资助项目(2011M500126
2012T50656)
关键词
金纳米棒
铅(Ⅱ)
荧光光谱法
四极子
Gold nanorods
Pb（ Ⅱ ）
Fluorospectrophotometric
G-quadruplex
作者简介
作者简介：甘浩（1988-），男，湖南长沙人，硕士研究生，主要研究方向为纳米材料在生物传感器上的应用。
