摘要
从酱香型白酒酒醅中分离到的30株放线菌中复筛出2株具有生物活性的菌株A2、A22,对2株放线菌代谢产物的生物学活性进行了比较研究,考察了放线菌发酵时间对其代谢生物学活性产物的影响,并且对其生物学活性物质进行了初步提取与生物活性检测。结果表明,放线菌A22、A2均能代谢产生生物学活性物质,A22代谢产物相对A2抑菌活性更强,在30℃、120 r/min摇床培养30~36 h时,代谢产物抑菌效果最强,初步结果表明此代谢产物极可能为一种非蛋白类和非肽类抗生素。
In this study, 30 Actinomycetes strains were isolated from fermented grains of Jiangxiang Baijiu(liquor). Then 2 Actinomycetes strains with antibacterial activities named A2 and A22 were obtained through secondary screening, and the effects of fermenting time on their metabolites were investigated, besides, their antibacterial substances were preliminary extracted and detected. The results suggested that, both A22 and A2 had antimicrobial activities, the metabolites of A22 had higher antibacterial activity, and its antibacterial activity was the strongest after 30~36 h shake cultivation at 30℃at the speed of 120 r/min. Its metabolites might be non-protein and non-peptide antibiotics.
出处
《酿酒科技》
2015年第8期1-5,10,共6页
Liquor-Making Science & Technology
基金
贵州省工业攻关项目(黔科合GZ字(2011)3015)
贵州省科技厅重大专项(黔科合重大专项字[2012]601-5号)
贵州科学院创新人才团队项目
关键词
微生物
酱香型白酒
放线菌
抑菌活性
白酒
microorganisms
Jiangxiang Baijiu(liquor)
Actinomycetes
antibacterial activity
Baijiu(liquor)
作者简介
尤小龙(1989-),男,湖北襄阳人,在读研究生,研究方向:发酵工程。
黄永光(1976-),男,贵州人,研究员,博士,硕士生导师,贵州省酿酒工业协会副秘书长,贵州省白酒专家委员会专家,贵州省白酒评委,主要从事白酒微生物及其酶、白酒风味及其功能因子等基础研究及其应用、酿酒工艺、酒类食品安全、行业产业政策研究及酿酒科技传媒工作,发表学术论文80余篇,E-mail:772566120@qq.com。