摘要
目的:探讨表皮生长因子受体(EGFR)抑制剂AG1478联合5-氟尿嘧啶(5-FU)对胆管癌细胞体外增殖、迁移和侵袭的影响。方法:体外培养人胆管癌QBC939细胞,分别用AG1478(10μmol/L)、5-FU(100 mg/L)以及联合两药处理24 h后,分别采用CCK-8法、划痕试验、Matrigel侵袭小室法检测细胞增殖、迁移及侵袭情况。结果:AG1478与5-FU单独作用后,QBC939细胞增殖、迁移和侵袭能力均明显降低(均P<0.05),而与任一单独用药比较,联合用药对细胞增殖、迁移和侵袭能力的抑制作用均明显增强(均P<0.05)。结论:EGFR抑制剂与5-FU联合应用可能是抑制胆管癌细胞生长、迁移和侵袭有效方法。
Objective： To investigate the effects of epidermal growth factor receptor （EGFR） inhibitor AG 1478 in combination with 5-fluorouracil （5-FU） on proliferation, migration and invasiveness of cholangiocarcinoma cells in vitro. Methods： Human cholangiocarcinoma QBC939 cells were cultured in vitro and exposed to AG1478 （10 μmol/L） and 5-FU （100 mg/L） alone or in combination for 24 h. The celt proliferation, migration and invasiveness were measured by CCK-8 assay, wound-scratch assay and Matrigel invasion chamber assay, respectively. Results： The abilities of proliferation, migration and invasion of QBC939 cells were significantly decreased after either lone AG1478 or 5-FU treatment （all P〈0.05）, and above effects were further enhanced by the combined treatment compared with either lone drug treatment （all P〈0.05）. Conclusion： Combination use of EGFR inhibitor and 5-FU may be an effective method for inhibiting the growth,migration and invasiveness of cholangiocarcinoma cells.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2015年第8期1102-1106,共5页
China Journal of General Surgery
基金
湖南省卫生厅医药卫生科研计划资助项目(B2012-088)
湖南省科学技术厅科技计划资助项目(2014FJ3033)
关键词
胆管肿瘤
受体
表皮生长因子
氟尿嘧啶
抗肿瘤联合化疗方案
Bile Duct Neoplasms
Receptor, Epidermal Growth Factor
Fluorouracil
Antineoplastic Combined Chemotherapy Protocols
作者简介
曾杰宏，湖南师范大学第一附属医院／湖南省人民医院硕士研究生，主要从事肝胆胰外科方面的研究。
通信作者：蒋波，Email：jiangbo@medmailmm．cn
