摘要
目的探讨Wnt3a在慢性牙周炎患者牙龈组织中的表达及其与附着丧失的关系。方法选择15例慢性牙周炎患者(牙周炎组)和15例牙周健康者(对照组),临床检查记录2组的探诊深度和附着丧失水平,采用RT-PCR和Western-Blot检测牙龈组织中Wnt3a的表达水平,并分析Wnt3a蛋白表达与附着丧失的相关性。结果牙周炎组的探诊深度高于对照组(mm:7.93±1.56 vs 1.83±0.37),其临床附着丧失为(7.76±1.34)mm,而对照组的口腔检查中未见临床附着。与对照组比较,牙周炎组的牙龈组织中Wnt3a m RNA(0.49±0.03 vs 0.36±0.04)和蛋白的表达水平(0.57±0.14 vs 0.43±0.17)均较低(t分别为12.015和2.381,P<0.05)。并且,附着丧失的程度与牙周炎患者牙龈组织中Wnt3a蛋白的表达呈负相关(r=-0.564,P=0.028)。结论 Wnt3a在慢性牙周炎中表达下调,与慢性牙周炎的附着丧失有密切关系,可能在慢性牙周炎的骨吸收中起到了一定的作用。
Objective To study the expression of Wnt3a in chronic periodontitis patients and its clinical significance. Methods Wnt3a expression was examined by RT-PCR and Western-Blot in 15 cases of chronic periodontitis gingival tissues and 15 normal tissues. Probe depth and attachment loss were also compared between these two groups. Correlation between Wnt3a expression and attachment lose were also analyzed. Results Probe depth was deeper in chronic periodonititis groups than that in control group（mm：7.93±1.56 vs 1.83±0.37）. Attachment loss was（7.76±1.34）mm in chronic periodontitis group while it was not seen in control gorup. Wnt3a was lower both in transcription level（0.49±0.03 vs 0.36±0.04）and expression level（0.57±0.14 vs 0.43±0.17, P〈0.05） in chronic periodonitis tissue compared with that in normal tissues. The higher clinical attachment loss was negatively correlated with expression of Wnt3a （r=-0.564,P=0.028）. Conclusion Wnt3a is expressed less in chronic periodontitis than that in control group. Wnt3a plays an important role in attachment loss and bone absorption.
出处
《天津医药》
CAS
2015年第8期883-885,共3页
Tianjin Medical Journal
基金
辽宁省自然科学基金项目(2013022024)
关键词
牙周炎
慢性病
牙龈
WNT蛋白质类
临床附着丧失
Wnt3a
periodontitis
chronic disease
gingiva
Wnt proteins
Wnt3a
clinical attachment loss
作者简介
程焕芝（1990-），女，硕士研究生，主要从事牙周病的预防和治疗方面研究
通讯作者E-mail：jzgaoxiuqiu1988@163．com
