摘要
建立一种利用高效液相色谱测定白茶中8种有机酸的方法,最佳色谱条件为：Agilent-1200高效液相色谱仪,ZORBAX SB-C18色谱柱（5μm,4.6 mm×250 mm）,流动相为3%甲醇-0.05 mol/L KH2PO4（用磷酸调p H至2.75）,流速0.5 m L/min,柱温25℃,检测波长215 nm,进样量15μL。该方法有机酸添加回收率达到91.63%~103.34%,相对标准差为0.45%~2.34%,有机酸线性相关系数r〉0.9997,表明该测定方法快速简便,具有较高的准确度和精确度。
This paper aimed to determine how to test 8 organic acids in white tea by using high performance liquid chromatography（HPLC）. The best chromatographic conditions are as follows： Agilent-1200 HPLC equipped with UV detector and a ZORBAX SB-C18column（5 μm,4.6 mm×250 mm）. The mobile phase was 3% methanol-0.05 mol/L KH2P04（p H value adjusted with phosphoric acid to 2.75） with flow rate of 0.5 m L/min. Column temperature was at 25 ℃, the detection wavelength was at 215 nm and 15 μL sample was injected into the column.The recovery rate of the organic acids could reach 91.63% ~103.34% with relative standard deviations of 0.45% ~2.34%. The organic acids linear correlation coefficient was above 0.9997, indicating that the method was simple,efficient and accurate.
出处
《中国茶叶加工》
2015年第3期37-42,共6页
China Tea Processing
基金
国家自然科学基金(31270735)
福建省现代农业(茶叶)产业技术体系项目(2060302闽财指[2012]0516号)
作者简介
何水平（1990-），女，福建南平人，在读硕士生，研究方向为茶叶加工与加工工程。
通讯作者：sunyunll25@126．com；
gcf104@163．com
