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依达拉奉对戊四氮致痫大鼠海马氨基酸递质通路的影响

Effects of edaravone on the amino acid neurotransmitter pathway from the hippocampus in pentylenetetrazol-induced epileptic rats
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摘要 目的探讨依达拉奉对戊四氮(PTZ)致痫大鼠海马氨基酸递质通路的影响。方法将36只成年Wistar大鼠随机分为正常对照组(NC组)、模型组(M组)和依达拉奉组(E组),观察3组大鼠的行为学和脑电图情况,应用免疫组织化学方法测定各组大鼠海马γ-氨基丁酸转运体和谷氨酰胺合成酶的含量。结果与M组相比,E组大鼠癫痫发作程度和脑电图改变以及海马γ-氨基丁酸转运体的含量均明显下降(P均<0.05),而谷氨酰胺合成酶的含量则明显升高(P<0.05)。结论依达拉奉可通过降低癫痫大鼠海马γ-氨基丁酸转运体含量和增强谷氨酰胺合成酶表达以达到对抗癫痫的效果。 Objective It is to investigate the effects of edaravone on the amino acid pathway from the hippocampus in pentylenetetrazol（ PTZ）-induced epileptic rats. Methods 36 adult Wistar rats were randomly divided into normal control group（ NC group）,epileptic model group（ M group） and edaravone group（ ET group）. The changes of behavior and EEG were observed in rats of the three groups. The expression of hippocampal γ-aminobutyric acid transporter and glutamine synthetase at protein level was detected by immunohistochemistry. Results Compared with that in M group,epileptic degree and EEG changes and the expression of hippocampal γ-aminobutyric acid transporter was significantly decreased（ P〈0. 05）,while the expression of glutamine synthetase was significantly increased in ET group（ P〈0. 05）. Conclusion Edaravone could ameliorate epilepsy via a decrease of the expression of hippocampal γ-aminobutyric acid transporter and an increase of the expression of glutamine synthetase in pentylenetetrazol（ PTZ）-induced epileptic rats.
作者 李飞 成祥林
机构地区 长江大学临床医学院 湖北省荆州市第一人民医院
出处 《现代中西医结合杂志》 CAS 2015年第23期2533-2535,共3页 Modern Journal of Integrated Traditional Chinese and Western Medicine
关键词 癫痫 戊四氮 依达拉奉 大鼠 epilepsy pentylenetetrazole edaravone rat
分类号 R332 [医药卫生—人体生理学]
作者简介 李飞，男，主治医师，研究生在读，研究方向为急危重症。 [通信作者]成祥林，E-mail：cheng066@tom．com
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参考文献14

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现代中西医结合杂志
2015年 第23期

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