摘要
目的探讨皮下注射异丙肾上腺素(isoprenaline,ISO)在诱导小鼠心肌肥厚模型中的作用。方法将雄性C57品系的小鼠随机分为药物组(ISO组)与生理盐水组(NS组),分别皮下注射剂量为5mg/kg的ISO与等体积的生理盐水,每日2次,注射2周后取材。用射血分数、心重体重比、肺重体重比、心肌细胞横截面积、和ANP表达量去验证心肌肥厚模型构建情况;Western blot法用于检测心肌肥厚相关信号通路改变情况。结果 ISO组与NS组相比,射血分数降低,心重体重比、肺重体重比升高、心肌细胞横截面积和ANP表达水平升高,证明ISO成功诱导了心肌肥厚模型;ISO组P-ERK、P-JNK与NS组相比表达增强,证明了ISO是通过MAPK家族信号通路诱导心肌肥厚模型。结论皮下注射异丙肾上腺素能够成功的通过激活MAPK家族信号通路诱导小鼠心肌肥厚模型,该法简单、经济、可靠。
Objective To investigate effects of subcutaneous injection of isoprenaline （ ISO） on cardiac hypertrophy in mice.Meth-ods Male C57 mice were divided into two groups：normal saline group （ NS group） and ISO group.The mice were subcutaneously injec-ted with a dose of 5mg/kg of ISO 2 times a day for two weeks, meanwhile mice in the NS group received the same volume of vehicle.The ejection fractions（EF%）were recorded after mice cardiac ultrasound.The heart weight（HW）, lung weight（LW）, and body weight（BW） were recorded and then the ratios of HW/BW and LW/BW were calculated.The cross-sectional areas （ CSA） of the myocytes was also counted.Atrial natriuretic peptide （ ANP） was detected by real time PCR.The signaling pathway involved in the cardiac hypertrophy was also detected by western blot.Results ISO induced reduced EF%,induced increased HW/BW, LW/BW, CSA of myocyte and mRNA level of ANP.And expressions of phospho-ERK and phospho-JNK in ISO group were enhanced compared with NS group.Conclusion Subcutaneous injections of ISO can effectively induce cardiac hypertrophy in mice model through the activation of MAPK signaling pathway.
出处
《医学研究杂志》
2015年第7期88-91,共4页
Journal of Medical Research
作者简介
通讯作者：唐其柱，教授、博士生导师，电子信箱：qztang@whu．edu．cn
