摘要
目的：检测低氧诱导因子（HIF-1α）、血管内皮细胞生长因子（VEGF）及CD34在膝骨关节炎（OA）软骨中的表达。方法60只Wister大鼠，鼠龄3个月，体质量约200～250 g。随机分为实验组45只和对照组15只。实验组行单膝前交叉韧带切断术，对照组不做处理，作为空白组对照；术后9周处死动物，采用免疫组织化学苏木精-伊红（HE）染色对实验组和对照组HIF-1α、VEGF、CD34表达进行检测。结果 HIF-lα、VEGF、CD34在实验组中阳性表达率分别为66.67%（30/45）、73.33%（33/45）、71.11%（32/45），均明显高于对照组；两组数据之间的差异有统计学意义（P〈0.05）。实验组HIF-1α、VEGF、CD34阳性细胞计数显著高于对照组（P〈0.05）；HIF-1α与VEGF、CD34表达存在正相关（r=0.249、0.289，P〈0.05）。结论 OA软骨中HIF-1α、VEGF及CD34高表达，HIF-1α与VEGF、CD34表达存在正相关，且HIF-1α、VEGF与CD34在OA软骨退行性变进展中共同发挥着重要作用。
Objective To observe the expression and significance of hypoxia inducible factor-1α（HIF-1α）, vascular endothelial growth factor（VEGF） and CD34 in osteoarthritis articular of rat model. Methods Sixty 3-month-old Wister rats with body weight 200-250 g were chosen and randomly divided into experimental group（n=45） and control group（n=15）. Rats in experimental group were performed single knee transaction of anterior cruciate ligament, while no treatment was performed in control group. Rats in experimental group and control group were sacrificed 9 weeks after operation, and the expressions of HIF-1α, VEGF and CD34 were detected by immunohistochemical hematoxylin-eosin（HE） staining. Results Positive expression rates of HIF-1α, VEGF and CD34 in experimental group was 66.67%（30/45）, 73.33%（33/45） and 71.11%（32/45）, respectively, which were significantly higher than those of control group, and there was statistically significant difference between 2 groups （P〈0.05）;Positive expression numbers of HIF-1α, VEGF and CD34 in experimental group were significantly higher than those of control group（P〈0.05）. It was showed positive correlation between HIF-1αand VEGF, CD34（r=0.249, 0.289, P〈0.05）. Conclusion It is demonstrated that of HIF-1α, VEGF and CD34 have high expression in rat model of osteoarthritis articular, and positive correlation exists between HIF-1αand VEGF, CD34, and they play important roles together in osteoarthritis carilage degeneration.
出处
《生物医学工程与临床》
CAS
2015年第4期407-411,共5页
Biomedical Engineering and Clinical Medicine
基金
国家自然科学基金(青年)资助项目(81301592)
作者简介
翁金清（1989-），男，湖北省人，硕士研究生，主要从事骨科研究。E-mail：1810164746@qq．com。
通讯作者：彭昊（1963-），男，湖北省人，博士，主任医师，教授，博导，主要从事骨坏死与骨关节炎的基础与临床研究。电话：027—88041911-82031。E-mail：13971482808@163．com。
