摘要
[目的]为墨吉明对虾的工厂化健康养殖提供理论依据。[方法]采用静水生物毒性法研究亚硝酸盐对墨吉明对虾的24、48、96 h急性毒性效应,分析96 h半致死浓度胁迫下墨吉明对虾血清蛋白含量、超氧化物歧化酶（SOD）活力以及溶菌酶活力的变化。[结果]亚硝酸盐毒性效应与亚硝酸盐浓度和胁迫时间呈正相关。亚硝酸盐对墨吉明对虾24、48、96 h的半致死浓度分别为281.83、151.36和114.82 mg/L,其安全浓度为11.48 mg/L。114.82 mg/L的亚硝酸盐对墨吉明对虾进行胁迫,在24-96 h范围内墨吉明对虾免疫指标随着胁迫时间的延长而发生显著变化,其中血清蛋白含量随着胁迫时间的延长而呈先升高后下降的趋势,且显著低于对照组（P〈0.05）。墨吉明对虾肝胰脏中超氧化物歧化酶（SOD）活力在48 h内呈下降趋势,且48 h SOD活力最小（148.90 U/mg prot）,而48 h后SOD活力逐步上升。在胁迫时间24-96 h范围内,肝胰脏中溶菌酶活力随着亚硝酸盐胁迫时间的延长而逐渐升高,96 h达到最大值（0.62U/ml）。墨吉明对虾血清蛋白含量、肝胰脏SOD活力和溶菌酶活力在3个取样时间点均存在显著差异（P〈0.05）,而对照组在3个取样时间点差异不显著（P〉0.05）,而在3个取样时间点对照组血清蛋白含量和2种酶的活力均未达到显著差异（P〉0.05）。[结论]墨吉明对虾对亚硝酸盐的安全浓度达到11.48 mg/L,证实该虾类对亚硝酸盐具有较高的耐受性。当水体中亚硝酸盐浓度超过安全浓度时,会导致墨吉明对虾的死亡。
[ Objective ] The research aimed to provide theoretical basis for the healthy industrial culture of Fenneropenaeus merguiensis. [ Method] The acute toxicity effects of nitrite to F. merguiensis in 24 h, 48 h and 96 h were studied by using the hydrostatic biological toxicity method. The changes of serum protein content, activities of SOD and lysozyme in F. merguiensis under the stress of 96 h half lethal concentration were analyzed. [ Result] The toxicity effect of nitrite showed positive correlations with nitrite concentration and stress time. The half lethal concentrations of nitrite to F. merguiensis were 281.83, 151.36 and 114.82 mg/L in 24 h, 48 h and 96 h respectively, and the safe concentration reached at 11.48 mg,/L. When F. merguiensis was stressed with 114.82 mg/L nitrite, the immune parameters of F. merguiensis had signifieant changes with the prolonging of stress time in the range of 24 - 96 h. The serum protein content showed the trends of firstly increasing and then decreasing with the prolonging of stress time, and it was significantly lower than that of control group （ P 〈 0.05 ）. SOD activity in the hepatopanereas of F. merguiensis showed the decreasing trend within 48 h, and SOD activity in 48 h reached the minimum value （ 148.90 U/mg prot） and SOD activity gradually increased. Within 24 -96 h（ stress time）, lysozyme activity in the hepatopanereas of F. mergu/ens/s gradually increased with the prolonging of stress time, and it reached the maximum value（0.62 U/ml） in 96 h. The serum protein content, activities of SOD and lysozyme in F. merguiensis had significant differences in three sampling time points （ P 〈 0.05 ）, while all the indices in control groups had no significant difference （ P 〉 0.05 ）. [ Conclusion ] The safe concentration of F. rnerguiensis to nitrite reached 11.48 mg/L,which confirmed this shrimp species possessed higher tolerance to nitrite. When the concentration of nitrite in water exceeded the safe concentration, the mass death of F. merguiensis would be caused.
出处
《安徽农业科学》
CAS
2015年第21期160-163,共4页
Journal of Anhui Agricultural Sciences
基金
广东省水产良种体系建设项目(2013004)
广西科学研究与技术开发计划项目(桂科攻14121006-2-6)
关键词
墨吉明对虾
亚硝酸盐
半致死浓度
免疫因子
Fenneropenaeus merguiensis
Nitrite
Half lethal concentration
Immune factors
作者简介
栗志民（1972-），男，辽宁抚顺人，副教授，博士，从事水产无脊椎动物增养殖研究。
通讯作者，高级实验师，硕士，从事水产经济动物增养殖及水域生态学研究。
