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茶树新品系铁香茗的SRAP亲缘关系分析

SRAP Relationship Analysis of New Tea Cultivar Tiexiangming
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摘要 茶树新品系铁香茗制茶具有天然的兰花香,但至今尚未明确其亲本来源。本文采用针对开放式阅读框扩增的SRAP标记中的21对引物,对铁香茗及选取的40个有代表性的茶树品种进行扩增,获得共81条带,其中多态性带数为77条,平均每对引物组合产生3.7条带,其多态性比率为60%-100%,平均为95.32%。铁香茗与金萱相似系数最近,达到0.8,并且与适制乌龙茶品种和来自福建的品种亲缘关系较近,据此推测,铁香茗的父母本或者一方来源于福建并且是适制乌龙茶的品种。 The tea made by new tea cultivar Tiexiangming carries natural orchid flavor, but its parental source is still unkown. This experiment used the 21 pairs of primers in SRAP molecular markers amplified from the open reading frame and 81 bands were obtained from the amplification of Tiexiangming and other 40 representing tea cultivars, among which 77 of them were polymorphic bands. Each pair of primers produce average 3.7 bands, and 60% ~100% of them were polymorphic, averaging 95.32%. The similarity coefficient between Tiexiangming and Jinxuan reach nearly 0.8. and it has closer relationship with the oolong tea cultivars and those from Fujian. It' s speculated that the parents or one part of its parents of Tiexiangming originated from Fujian or oolong tea cultivars.
出处 《茶叶通讯》 2015年第2期7-14,共8页 Journal of Tea Communication
基金 国家自然科学基金项目(31271789) 教育部博士点基金(20124320110014) 湖南省教育厅项目(12C0183)
关键词 茶树 铁香茗 SRAP 亲缘关系鉴定 tea tree, Tiexiangming, SRAP, parentage identification
作者简介 郭嘉懿(1988-),男,天津人,在读硕士研究生,研究方向:茶树栽培育种及分子生物学。 通讯作者:scw69@163.com
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参考文献1

  • 1Li G,Quiros C F.Sequence2related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica [J].Theoretical and Applied Ganetics,2001,103: 455-461.

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