摘要
从高粱Zn缺乏处理的深度测序数据中,得到一个差异表达显著的基因Sb04g031980,序列分析表明,该基因编码一个MATE家族转运蛋白,故命名为SbMATE。为揭示该基因在高粱Zn响应中的功能,将SbMATE基因置于酵母表达启动子之下,构建酵母表达载体,并将其转入重金属敏感型酵母突变体。酵母中亚细胞定位分析表明,SbMATE定位于酵母细胞的液泡中。酵母重金属耐受性实验表明,SbMATE在酵母中的过量表达提高了酵母细胞对Zn2+毒害的耐受性,表明SbMATE在植物细胞对Zn2+吸收和耐性中发挥了重要作用。
A Zn-responsive gene, Sb04g031980, was obtained by the deep sequencing of differential gene expression analysis in Sorghum bicolor. The sequence analysis showed that the gene encoded a MATE subfamily transporter, named SbMATE. For elucidating the function of the gene, the yeast-expressed plasmids with SbMATE were constructed and transferred into heavy metal sensitive yeast mutant ceils. The subcellular localization analysis by the fused-GFP protein expression showed that SbMATE was localized in vacuolar of yeast cell. Further study about the growth pattern of Sb- MATE-expressed yeast indicated that overexpression of SbMATE improved Zn2＋ toxicity resistance in the yeast cells, suggesting that SbMATE plays a role in Zn accumulation and tolerance in plant.
出处
《山西农业大学学报（自然科学版）》
CAS
2015年第4期342-349,共8页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
国家自然科学基金(31170228
31272239)
河北省自然科学基金杰出青年基金(C2013503042)
荒漠与绿洲国家重点实验室基金(2013-01-01)
关键词
高粱
MATE
基因克隆
酵母表达
Sorghumbicolor
MATE
Gene cloning
Yeast expression.
作者简介
张苹（1987-），女（汉），山东济南人，硕士，研究方向：植物分子生物学
通讯作者：王文颖，教授。Tel：0871-65140420；E-mail：1714565575@qq．com
通讯作者：徐进，研究员。Tel：0871-65140420；E—mail：xujin@xtbg．ac．cn
