摘要
利用愈创木酚选择培养基,从11种白腐菌株中定性筛选出3株白腐菌,分别是毡毛栓孔菌Trametes velutina Dai10149、鍺栓孔菌T.ochracea Cui 6888、绒毛栓孔菌T.pubescens Cui 7571。重量法绘制它们的生长曲线,同时测定其蛋白质含量及胞外酶活。通过比较发现,绒毛栓孔菌T.pubescens Cui 7571的生长速度快,且具有较强及稳定的酶活分泌能力;对针叶植物、阔叶植物及单子叶草本的木质纤维素均表现出较好的降解效果。
Based on testing of guaiacol selective medium, 3 isolates from 11 white-rot fungi were selected, and they areTrametes velutina Dai 10149,T. ochraceaCui 6888 and T. pubescens Cui 7571. Then growth curve of the 3 isolates based on weight method were plotted, and the changes of protein content and two extracellular enzyme activities were monitored. The results showed thatT. pubescens Cui 7571 had excellent ability of growth, and stable and outstanding ability of secreting cellulase and laccase. It can significantly decompose the lignocelluloses in conifer, hardwood and monocotyledon plants.
出处
《生物技术通报》
CAS
CSCD
北大核心
2015年第6期151-156,共6页
Biotechnology Bulletin
关键词
白腐菌
生长曲线
酶活
组分含量
降解能力
white-rot fungi
growth curve
extracellular enzyme activity
chemical composition content
degradation capacity
作者简介
吴雪君，女，硕士，研究方向：森林病理学；E—mail：xuejunw293@163．com
通讯作者：崔宝凯，男，教授，研究方向：菌物系统学；E-mail：baokaicui@yahoo．com．cn
