摘要
为了研究牦牛肠源Lactobacillus acidophilus L3（嗜酸乳杆菌）对其肠道分泌型免疫球蛋白A（SIg A）及免疫相关因子的影响,将10头健康牦牛（2～2.5岁）随机分为2组,分别为益生菌组和空白对照组,益生菌组在饲料中添加2×109CFU·kg-1L.acidophilus L3,饲喂28 d后取其小肠样品,ELISA检测试验组和对照组十二指肠、空肠、回肠中SIg A、白细胞介素-2（IL-2）、IL-4、IL-6、干扰素γ（IFN-γ）的含量。研究结果表明,L.acidophilus L3可有效增加试验组牦牛肠道SIg A的分泌量（P〈0.05）,组内比较发现SIg A在回肠含量最高,其次为空肠和十二指肠。IL-2、IL-4、IL-6在试验组小肠中的表达量较对照组显著增加（P〈0.05）,试验组小肠IFN-γ的表达量较对照组降低（P〈0.05）。证实L.acidophilus L3可提高牦牛肠道的黏膜免疫功能。
In order to study the effects of Lactobacillus acidophilus L3 on the intestinal SIgA and immune factors of yaks, 10 yaks aged from 2 to 2.5 years were used in this study, and 5 of which were fed daily with L. acidophilus L3 （ a concen- tration of 2 × 10^9CFU · kg^-1 feed）, while the others were conventional feed without adding L. acidophilus L3. Small intesti- nal samples were collected on day 28, and the content of SIgA, IL-2, IL-4, IL-6, IFN-γ in duodenum, jejunum, and ileum were detected by ELISA. The results showed that the content of intestinal SIgA was significantly increased in the probiotic group compared with the control （ P 〈 0.05 ）. The highest content of SIgA was found in ileum followed by jejunum and duo- denum. The content of intestinal IL-2, IL-4 and IL-6 were also significantly increased in the probiotic group compared with the control （P 〈 0.05）. The content of intestinal IFN-γ was decreased in the probiotic group compared with the control （ P 〈 0.05 ）. These results indicated that L. acidophilus L3 can effectively improve the intestinal mucosal immune function of yaks by increasing the levels of intestinal SIgA, IL-2, IL-4 and IL-6.
出处
《四川动物》
北大核心
2015年第3期411-414,共4页
Sichuan Journal of Zoology
基金
陕西省教育厅科学研究计划(自然科学专项)项目2013JK0721
陕西理工学院人才启动院项目SLGQD13-19
陕西理工学院人才启动院项目SLGQD14-01
农业部兽用药物创制重点实验室
甘肃省新兽药工程重点实验室开放课题1610322011011
作者简介
祁珊珊（1984－），女，博士，讲师，主要从事动物病理学研究，E-mail：qishanshan101@126．com
博士，讲师，主要从事药理毒理学研究，E-mail：zhenghongxing100@126．com
