摘要
以低聚木糖作为羰基供体与脯氨酸进行美拉德反应,监测反应过程中p H、紫外-可见吸收值以及荧光值,提取反应5 h、15 h以及30 h的低聚木糖衍生物XP-5h、XP-15h和XP-30h。对3种衍生物进行红外表征和相对分子质量测定,并研究其对DPPH自由基、超氧阴离子自由基(O-2·)的清除能力以及还原能力。结果表明:低聚木糖衍生物对DPPH自由基、O-2·的清除能力以及还原能力均较低聚木糖得到显著提高;XP-30h的抗氧化性最强,XP-15h次之,XP-5h最弱,即随着反应的进行低聚木糖衍生物的抗氧化性能增强。
Maillard reaction was occurred by heating xylo-oligosaccharide （XO） with proline. The pH, UV-Vis absorbance and fluorescence were determined during the reaction. Three kinds of xylo-oligosaccharide derivatives were extracted and named as XP-5h, XP-15h and XP-30h. Xylo-oligosaccharide derivatives were characterized by FTIR and their molecular weight was determined by GPC. Their antioxidant activity was investigated through various estab- lished in vitro systems, such as its ability to scavenge and reduce 1,1-diphenyl-2-pivrylhydrazyl （DPPH） and superox- ide anion radicals. The results indicated that three kinds of xylo-oligosaccharide derivatives showed stronger antioxi- dant activities compared with xylo-oligosaccharides. The antioxidant activities were found to be in the order of XP-30h 〉 XP-15h 〉 XP-5h, which indicated that the antioxidant activity of xylo-oligosaccharide derivatives was enhanced dur- ing the process of the reaction.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第1期18-22,共5页
Food and Fermentation Industries
基金
"十二五"国家支撑计划项目"鲜活农产品安全低碳物流技术与配套装备"
淡水水产品保活冷鲜冷链物流关键技术研发(2012BAD38B04)
关键词
低聚木糖
脯氨酸
美拉德反应
抗氧化活性
xylo-oligosaccharide
proline
Maillard reaction
antioxidant activity
作者简介
硕士研究生
（孙涛副教授为通讯作者，E-mail：taosun@shou．edu．cn）。
