摘要
目的:研究环境污染物苯并芘(Ba P)致人绒毛膜滋养层细胞HTR8-SVneo氧化损伤的作用机制,探讨五味子乙素(Sch B)的可能保护作用。方法:本实验分为空白组、Ba P组、Sch B不同浓度组(0.1,0.5,2.0μmol/L),以HTR8-SVneo细胞为载体,构建Ba P氧化应激损伤模型,测定氧化和抗氧化指标。结果:与空白组比较,Ba P组超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的浓度显著降低,而乳酸脱氢酶(LDH)、丙二醛(MDA)、一氧化氮(NO)和诱导型一氧化氮合酶(i NOS)的浓度明显增加(P<0.05)。不同剂量Sch B组SOD、GSH-Px的浓度明显提高,LDH、MDA、NO、i NOS的浓度减少,与Ba P组比较,差异有统计学意义(P<0.05)。结论:Sch B能调节Ba P所致的HTR8-SVneo细胞氧化系统和抗氧化系统的失衡,从而预防Ba P致HTR8-SV neo细胞的氧化损伤。
Objective： To study the oxidative damage of benzo[a]pyrene（BaP） in HTR8-SVneo cells, and to explore the protective effect of Schizandrin B （Sch B）. Methods：The HTR8-SVneo cells were in vitro cultured, and the oxidative stress damage model was induced by BaP of different doses （0.1,0.5,2.0 μmol/L）. The experiment were designed as the control group, the BaP group, and the Sch B （0.1,0.5,2.0 μmol/L） groups. The oxydic parameters and antioxidative indexes were detected. Resets：Compared with control group,the BaP group had significantly lower levels of GSH-Px and SOD and higher levels of LDH,MDA,NO and iNOS （P〈 0.05）. The Sch B groups （0.1,0.5,2.0 μmol/L） had significantly higher levels of SOD and GSH-Px and lower levels of LDH, MDA,NO and iNOS than the BaP group （P〈0.05）. Conclusions：Sch B can rebalance between oxidation and anti-oxidation in the HTRS-SVneo cells treated by BaP, suggesting the protective effects of Sch B on oxidative damage.
出处
《国际生殖健康／计划生育杂志》
CAS
2015年第3期181-183,共3页
Journal of International Reproductive Health/Family Planning
基金
国家自然科学基金(81273977)
天津市自然科学基金(12JCYBJC16200)
作者简介
通信作者：陈亚琼，E-mail：chenyq82@hotmail．com
