摘要
为了评估细角螺苗种对低盐胁迫的耐受能力,将细角螺(Hemifusus ternatanus)幼螺养殖盐度自27逐渐降低至22、18和14,测定处理后不同时间细角螺幼螺的耗氧率和排氨率。结果显示,盐度27降至22后幼螺耗氧率先显著降低,在第二天升至对照组水平,此后略有波动但均与盐度27无显著差异;盐度降至18与对照组无显著性差异;盐度降至14,细角螺的耗氧率先有显著升高,后与对照组相比无显著差异。盐度从27降至22后,幼螺的排氨率在第一、第二天显著升高,其后逐渐降低,盐度自22降至18和14,排氨率呈先降低后升高的趋势。盐度22处理后幼螺的氧氮比先降低后升高,盐度18和14处理组先升高后逐渐降低,说明低盐胁迫影响细角螺幼螺能量物质分解代谢的比率,胁迫条件下幼螺以脂肪和碳水化合物为主要供能物质。实验结果表明,第一次低盐处理对幼螺呼吸排泄的影响较大,此后再逐渐降低盐度,幼螺具有一定的适应能力。本实验结果可为细角螺养殖和放流前盐度驯化提供参考。
Hemifusus ternatanus is a predatory marine whelk inhabiting in the coast of eastern and southern China and Japan. They are sold as luxurious food on markets due to rich nutritional contents of essential amino acids and unsaturated fatty acids. But overfishing has resulted in serious natural stock depletion of this species. Artificial breeding and aquaculture technologies have been studied to develop a new aquaculture species and help to recover natural resources. Coastal shellfish frequently suffers from salinity fluctuations due to weather changes or human activities,especially low-salinity stress. It was reported that low-salinity stress would affect the growth, ingestion, respiration, heart beating rate and other physiological activities of shellfish,and even cause death. In the coast of southern and eastern China,seawater salinity is frequently reduced to about 15,but now little is known about the physiology and environmental adaptation of H.ternatanus. Therefore,it is necessary to evaluate the tolerance of H. ternatanus to low salinity.To assess the tolerance of H. ternatanus to low-salinity stress,the seawater salinity was gradually decreased from 27 to 22,18 and 14. The respiration and excretion rates of juveniles after low-salinity challenge were measured. Results showed that from salinity 27 to 22,the respiration rate was significantly lower than that of the control group and then rose to the control level on the second day. When salinity was decreased from 22 to 18 and from 18 to 14,the respiration rate rose slightly and no significant difference was observed with the control group. When salinity was decreased from 27 to 22,the ammonia excretion rate increased significantly in the first two days,and then gradually declined. During 22- 18 and 18- 14 treatment,the excretion rate declined at first and then rose. After transferred to salinity 22,the O / N ratio decreased first and then increased,contrary to the variation in salinity 18 and 14 groups,suggesting that lowsalinity stress affected the catabolism ratio of energetic compounds,and fat and carbohydrate were catabolized firstly to provide energy under salinity stress conditions. Results indicated that the first decrease of seawater salinity had a greater impact on juveniles＇ respiration and excretion; after that,the juveniles showed certain adaptability. The experiment results can provide reference for salinity acclimation before aquaculture and seedling releasing.
出处
《海洋渔业》
CSCD
北大核心
2015年第3期259-263,共5页
Marine Fisheries
基金
中国水产科学研究院基本科研业务费项目(2013A07XK03)
关键词
细角螺
幼螺
耗氧率
排氨率
氧氮比
Hemifusus ternatanus
juvenile
oxygen consumption rate
ammonia excretion rate
O/N ratio
作者简介
唐保军（1975-），男，博士，助理研究员，从事贝类繁殖生物学研究。E—mail：bjtang@yeah．net
