摘要
为了揭示极低频脉冲电场对作物抗旱性的影响及其机理,采用渗透势为-0.1 MPa的PEG-6000溶液模拟干旱条件,研究脉冲频率为1Hz、场强为200kV/m、脉宽为80ms的极低频脉冲电场对干旱胁迫下玉米幼苗根系延迟光子辐射动力学参数的影响.结果表明,在干旱胁迫下,玉米幼苗根系细胞延迟光子辐射动力学参数的初始光子数I0、相干时间τ和衰减参数β均随着胁迫时间的延长呈现出波动性的下降趋势,经过极低频脉冲电场处理的玉米幼苗根系细胞的初始光子数I0、相干时间τ和衰减参数β的变化趋势与之类似,但是,各动力学参数的数值均明显高于对照.延迟发光动力学参数的变化暗示极低频脉冲电场处理提高了渗透胁迫下玉米幼苗根系细胞的代谢水平和组织序性,增强了细胞内功能分子之间的相互作用.延迟光子辐射的分析结果为在活体细胞层面阐明极低频脉冲电场对作物抗旱性的影响及其机理提供了参考.
Extremely low frequency pulsed electric field could arouse a comparatively intense reaction on physiological characteristics of plant cells, so the effect of the extremely low frequency pulsed electric field on crops drought resistance is very worthy to be studied. The delayed biophoton emission of cells, as a kind of life information, can reflect the changes of cellular physiological state. In order to study the influence of the extremely low frequency pulsed electric field on drought resistance of plants seedlings and its mechanism, the PEG-6000 solution with the osmotic potential of --0. 1 MPa was used to simulate drought conditions, the germinating maize was treated by the extremely low frequency pulsed electric field at 100 kV/m, frequency 1 Hz and pulse width 80 ms, the changes of the delayed biophoton emission from maize seedling roots under osmotic stress were discussed. The results show that the changes of the kinetic parameters about delayed biophoton emission such as the initial photon number I0, coherence time r and decay factor β decrease volatility. The changes of the kinetic parameters about delayed biophoton emission of maize seedling root treated by extremely low frequency pulsed electric field are the same, but, the value of these parameter increase significantly in comparison with the control. The analysis indicates that the extremely low frequency pulsed electric field treatment can raise the level of the metabolic level of cell and improve tissue order, the interactions between functional molecules in cells is enhanced. The results of this paper provide a reference for explaining clearly the effect of extremely low freauencv pulsedelectric field on drought resistance of plants seedlings and its mechanism.
出处
《光子学报》
EI
CAS
CSCD
北大核心
2015年第5期155-160,共6页
Acta Photonica Sinica
基金
国家自然科学基金(Nos.51277151
31471412)
西安理工大学科技创新计划项目(No.2013CX019)资助
关键词
延迟光子辐射
脉冲电场
渗透胁迫
根系细胞
细胞代谢
组织序性
抗旱性
Delayed biophoton emission
Pulsed electric field
Osmotic stress
Root cell
Cellmetabolism
Tissue order
Drought resistance
作者简介
贺瑞瑞（1991-），女，硕士研究生，主要研究方向为生物电磁学和生物光子学，Email：heruiruijy@163．com
导师（通讯作者）：习岗（1957-），男，教授，主要研究方向为生物电磁学与生物光学，Email：xig@xaut．edu．cn
