摘要
目的：观察胃癌组织中微小RNA-181a（miR-181a）与其靶基因共济失调-毛细血管扩张突变基因（ATM）的表达与异常情况，初步研究其意义。方法收集2009年4月~2011年12月广州市第一人民医院外科手术切除的胃癌组织标本9例，同时选取其癌旁非癌组织标本作为对照，分析胃癌组织中miR-181a与ATM蛋白表达的相关性。提取其蛋白质及总RNA，采用实时荧光定量PCR（qRT-PCR）检测标本的miR-181a，Western blot检测ATM蛋白。比较胃癌组织及癌旁非癌组织中miR-181a、ATM蛋白表达量。结果 miR-181a在胃癌组织中的表达量为（1.981±1.800），miR-181a在邻近非癌组织中的表达量为（0.394±0.093）；灰度值检测：癌组织ATM蛋白为（0.2539±0.0046）；非癌组织为（0.5525±0.0660），癌及非癌组织中miR-181a、ATM蛋白表达量比较，差异均有高度统计学意义（P〈0.01）；miR-181a与ATM蛋白表达呈负相关（r=-0.539，P〈0.01）。结论 miR-181a在胃癌组织中表达明显增高，ATM蛋白表达明显下降；究其原因可能是通过基因沉默机制miR-181a降低ATM蛋白表达量，从而在胃癌的发生及发展中起促进作用。
Objective To investigate the expression and significance of microRNA （miRNA）-181a （miR-181a） and its target gene ATM in gastric carcinoma tissues. Methods Tissues of gastric carcinoma and adjacent non-tumorous were collected respectively from 9 patients given surgical operation in Guangzhou First People＆#39;s Hospital from April 2009 to December 2011. The total RNA and protein were extracted routinely, the miR-181a was detected by Real-time quanti-tative PCR, ATM protein was detected by Western blot. The expression of miR-181a and ATM protein between gastric carcinoma and adjacent non-tumorous were compared, and the correlation between miR-181a and ATM protein levels in gastric carcinoma was analyzed. Results In gastric carcinoma tissues, the expression of miR-181a and ATM protein were （1.981±1.800）, （0.2539±0.0046） respectively, whereas in the adjacent non-tumorous tissues, the expression of miR-181a and ATM protein were （0.394±0.093）, （0.5525±0.0660） respectively. The differences between the expression of miR-181a and ATM protein in these two tissues were statistically significant （P〈0.01）. There was a negative corre-lation between miR-181a and ATM protein levels （r=-0.539, P〈 0.01）. Conclusion The expression of miR-181a is significantly up-regulated in gastric carcinoma tissues, whereas the ATM protein is markedly decreased.miR-181a may inhibit ATM expression by post-transcriptional gene silencing to restrain gastric carcinoma occurrence and progress.
出处
《中国医药导报》
CAS
2015年第15期24-27,32,共5页
China Medical Herald
