摘要
以草菇(Volvariella volvacea)乙酰木聚糖酯酶AXE、AXEII为研究对象,采用生物信息学方法对其核苷酸及编码的蛋白氨基酸序列、组成成分、信号肽、跨膜结构域、疏水性/亲水性、蛋白质三级结构等进行预测和分析,并构建了乙酰木聚糖酯酶蛋白家族的系统进化树。结果表明,草菇的AXE蛋白由349个氨基酸残基组成,编码蛋白质的分子量为37.55 ku,等电点为8.58,属稳定性蛋白质。AXEII由253个氨基酸残基组成,编码蛋白质的分子量为27.70 ku,等电点为5.82,属稳定性蛋白质。两者均为亲水性蛋白,存在信号肽,AXE与AXEII的潜在磷酸化位点总数分别为15个和11个。草菇的AXE与灰盖鬼伞(Coprinopsis cinerea)亲缘关系较近,AXEII与裂褶菌(Schizophyllum commune)亲缘关系较近。
Acetyl xylan esterase（AXE and AXEII） from Volvariella volvacea was predicted and analyzed adopting bioinformatics,including nucleotide and amino acid sequences of code,signal peptide,trans-membrane topological structure,hydrohobicity or hydrophilicity,tertiary protein structure etc.A phylogenetic tree of acetyl xylan esterase family was also established.The results showed that AXE protein of V.volvacea consisted of 349 amino acid residues.The molecular weight of code protein was 37.55 ku and isoelectric point was 8.58 belonging to stable protein.AXEII consisted of 253 amino acid residues.The molecular weight of code protein was 27.70 ku and isoelectric point was 5.82 belonging to stable protein.Both of them were hydrophilic protein,existing signal peptide.The sum of potential phosphorylation site was 15 and11 respectively.Phylogenetic analysis indicated that AXE in V.volvacea was close in genetic relationship to Coprinopsis cinerea,however,AXEII in V.volvacea was close in genetic relationship to Schizophyllum commune.
出处
《微生物学杂志》
CAS
CSCD
2015年第2期53-58,共6页
Journal of Microbiology
基金
上海市科技兴农重点攻关项目(沪农科攻字(2011)第1-2号)
上海市市级农口系统青年人才成长计划(沪农青字(2014)第1-17号
上海市科技兴农推广项目(沪农科推字(2014)第2-2号)
关键词
草菇
乙酰木聚糖酯酶
生物信息学
Volvariella volvacea
acetyl xylan esterase
bioinformatics
作者简介
作者简介：赵妍女，博士，助理研究员。主要从事食用菌生理生化研究。Tel：021-37196813，E-mail：jiandan289@126．com
通讯作者。陈明杰男，博士，研究员。主要从事食用菌遗传育种研究。Tel：021-62200747，E-mail：mjchen@saas．sh．cn
通讯作者。王孟兰女，硕士，助理农艺师。主要从事食用菌分子生物学研究。Tel：025-68173932，E-mail：wm1245@126．com
