摘要
目的:研究急性高山病(AMS)发生及低氧运动习服中血清ET-1水平的变化趋势及ET-1基因多态与其水平的关系。方法:Phase 1:模拟海拔4 800 m低氧环境,49名北方汉族大学生急性暴露6 h,入舱30min后以恒定负荷蹬车20 min,常氧安静(NMⅠ)和急性低氧暴露结束(HYⅠ)时测定血清ET-1水平。Phase 2:恢复1周后,48名受试者在模拟环境下进行3周渐进式低氧训练。恢复1周,重复Phase 1的低氧暴露和运动,常氧安静(NMⅡ)和急性低氧暴露结束(HYⅡ)时测定ET-1水平。PCR-RFLP法检测受试者ET-1基因T8000C及G5665T位点基因型和等位基因频率。结果:1)低氧暴露后受试者的ET-1水平上升不显著,AMS组与非AMS组间的ET-1变化量△1(HYⅠ-NMⅠ)差异也不显著。3周低氧训练后,低氧习服与未习服组的ET-1水平在再次低氧暴露后均上升,上升幅度△4(HYⅡ-NMⅡ)均比初次暴露时(△3(HYⅠ-NMⅠ))增多,但仅低氧习服组的ET-1水平和△4上升显著。2)G5665T及T8000C位点不同基因型和等位基因携带者的AMS发生率及AMS评分变化趋势差异均不显著。低氧暴露后,G5665T/GG和T8000C/TT组的ET-1水平上升,G5665T/GT+TT和T8000C/TC+CC组的ET-1水平下降,但各组的ET-1水平变化及组间的ET-1变化量△1(HYⅠ-NMⅠ)差异均不显著。3周低氧训练后,各基因型组的ET-1水平在低氧暴露后均比NMⅡ时上升,变化幅度△4(HYⅡ-NMⅡ)均比初次暴露后(△3(HYⅠ-NMⅠ))增加,但差异均不显著。结论:1)血清ET-1水平对低氧不敏感,ET-1变化量△1不能作为AMS易感性辅助评价指标。3周低氧训练后,低氧习服与未习服组的ET-1水平在再次低氧暴露后变化程度不同,导致两组低氧习服效果迥异。2)G5665T和T8000C位点不是AMS易感及低氧习服的遗传学标记。G5665T与T8000C位点不同基因型组的ET-1水平在初次低氧暴露时变化趋势不同,在再次低氧暴露时变化趋势相同,但ET-1水平变化仅与低氧有关,与基因多态无关。
Objectives： The purpose of this study was to explore the change trend of serum ET-1 level in acute mountain sickness （AMS） and sports hypoxia acclimation, and explore the correlation between ET-1＇s gene polymorphisms and its level in serum. Methods： Phase 1 ： Forty-nine male Han nationality college students were involved in a 6 h acute hypoxia exposure with 4 800m altitudeequivalent. They rode bicycle at constant work rate for 20min after 30min adaptation to the hypoxia environment. Serum ET-1 levels were analyzed at state of normoxic resting （ NMⅠ） and the end of acute hypoxic exposure （ HYⅠ）. Phase 2 ： After one week recovery, 48 volunteers participated in 3 weeks hypoxia training （IAE） and one week recovery, finally they repeated the Phase I. ET-1 levels were analyzed at NMⅠI and HYⅠI, genotypes and alleles frequencies of ET-1 at T8000C and G5665T loci were measured by PCR-RFLP. Results ： 1 ） After hypoxia exposure, ET-1 didn＇ t increase significantly, and the a 1 （ HYⅠ - NMⅠ ） between AMS and non-AMS groups didn＇ t change. After 3 weeks hypoxia training, ET-1 of the hypoxia acclimatization group （HAG） and the hypoxia unacclimatization group （HUAG） increased after the second hypoxia exposure. The ET-1 increase of A4 （ HYⅡ - NMⅡ ） was higher than that of A3 （ HYⅠ - NMⅠ ）, but it was only happened significantly in HAG. 2 ） Occurrence rate and evaluation trend of AMS in different genotype and allele carriers at G5665T and TS000C loci didn＇ t change. After the hypoxia exposure, ET-1 level increased in G5665T/GG and T8000C/TT groups but decreased in G5665T/GT ＋ TT and T8000C/TC ＋ CC groups, the ET-1 level and ET-1 △ 1 had no differences between different groups. After 3 weeks hypoxia training, ET-I levels of the different genotype carriers increased after the second hypoxia exposure, and △4 （ HY Ⅱ - NMⅡ ） was higher than △3 （ HYⅠ - NMⅠ ） without significance. Conclusions ： 1 ） Serum ET-1 is not sensitive to hypoxia and △ 1 cannot be used as an assisted indicator to evaluate AMS predisposition. After 3 weeks hypoxia exposure, the ET-1 changes of HAG and HUAG are different, which led to the different hypoxia acclimatization effects between two groups. 2） G5665T and TS000C loci are not genetic marks of AMS predisposition and hypoxia acclimation. The ET-1 change of the different genotype carriers is different in the first hypoxia exposures, but is similar in the second hypoxia exposure. ET-1 change is associated with hypoxia rather than gene polymorphisms.
出处
《北京体育大学学报》
CSSCI
北大核心
2015年第4期58-64,共7页
Journal of Beijing Sport University
基金
国家自然科学基金项目(项目编号:31071032)
作者简介
周文婷，讲师，博士，北京体育大学博士后，研究方向运动员分子选材与高原训练。
