摘要
为奶牛隐性乳房炎主要致病菌的检测提供参考,对采集于蒙自市奶牛养殖户的10头疑似乳房炎患病奶牛新鲜奶样20份,采用传统细菌分离鉴定方法和PCR技术鉴定奶牛隐性乳房炎的链球菌。结果表明:从传统方法分离菌中提取DNA,经PCR扩增测序为1 541bp,与GenBank数据库中收录的乳房链球菌(登录号:JF896457.1)的序列相似性达80.8%,且与Streptococcus uberis USC71-LHICA菌株的亲缘关系最近,相似性达100%,由此表明,分离菌为乳房链球菌。与传统的细菌培养方法确定细菌种类相比,PCR方法检测具有快速、准确、灵敏度高等特点。
S.uberis of 20 fresh milk samples collected from 10 suspected mastitis cows were isolated and identified by traditional bacterial isolation and identification method and PCR technology to provide a reference for detection of main pathogenic bacteria of cow recessive mastitis.The results showed that the amplification sequence of DNA extracted from isolates is 154 bp,the sequence similarity between sequence of DNA extracted from isolates and S.uberis JF896457.1 in GenBank is up to 80.8%,and the similarity between sequence of DNA extracted from isolates and S.uberis USC71-LHICA strain is up to 100%, which indicates that isolates are S.uberis.PCR detection technology has the feature of rapidity,accuracy and high sensitivity compared with traditional bacterial cultural method.
出处
《贵州农业科学》
CAS
2015年第4期150-152,157,共4页
Guizhou Agricultural Sciences
基金
云南省科技厅应用基础研究面上项目"奶牛隐形乳房炎分子诊断试剂盒研制及综合防治对策研究"(2010ZC151)
作者简介
谢昆（1975-），男，副教授，博士，从事动物生物化学与分子生物学研究。E-mail：xk_biology2@126．com
