摘要
目的:研究九龙藤总黄酮(Bauhinia championii Benth flavones,BCF)对大鼠心肌缺血再灌注损伤后自由基代谢及细胞凋亡的影响。方法:将实验动物随机分为6组:假手术组、心肌缺血再灌注模型组、九龙藤总黄酮(30、60、120和240mg/kg)预处理组、复方丹参滴丸(135mg/kg)阳性对照组,术前1周开始灌胃给药。测定各组大鼠血清中谷草转氨酶(AST)、肌酸激酶(CK)、乳酸脱氢酶(LDH)活性、丙二醛(MDA)含量以及总抗氧化能力(T-AOC);检测心肌组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性以及丙二醛(MDA)含量;通过HE染色方法观察各组大鼠心肌组织病理学改变,并通过TUNEL染色观察细胞凋亡状况。结果:与模型对照组相比,九龙藤总黄酮(60、120和240mg/kg)预处理组大鼠血清中AST、CK、LDH活性显著降低,MDA含量显著升高,T-AOC显著升高;九龙藤总黄酮(60、120和240mg/kg)预处理组心肌组织中SOD、GSH-Px、CAT活性显著升高、MDA含量显著降低;心肌组织病理性改变和细胞凋亡程度均显著较轻。其中以240mg/kg组各指标变化最显著。结论:九龙藤总黄酮能够有效增强抗氧化酶活性,提高自由基清除能力,改善组织病理学改变,抑制细胞凋亡,提示九龙藤总黄酮对大鼠心肌缺血再灌注损伤具有剂量依赖性的保护作用。
Objective： To investigate the effects of Bauhinia championii Benth flavones（ BCF） on free radical metabolism and myocardial apoptosis after myocardial ischemia-reperfusion injury in rats. Methods： The rats were randomly devided into six groups： sham operation group,myocardial ischemia-reperfusion control group,BCF 30,60,120 and 240 mg / kg per-treated groups,and Fufangdanshendiwan（ 135 mg / kg） treated group. One week before operation,drugs were begin given by intragastrical. The T-AOC and the content of AST,CK,LDH,MDA in serum were determined; the activity of SOD / GSH-Px / CAT and the content of MDA in myocardial tissue were determined; the histopathological changes and myocardial apoptosis were observed. Results： Compared with myocardial ischemia-reperfusion control group,the T-AOC in serum was significantly increased and the content of AST,CK,LDH,MDA in serum were significantly decreased of BCF 60,120,240 mg / kg pertreated groups（ P 〈0. 05,P 〈0. 01）; the activity of SOD / GSH-Px / CAT in myocardial apoptosis of BCF 60,120,240 mg / kg per-treated groups groups were significantly increased（ P 〈0. 05,P 〈0. 01）,while the content of MDA were significantly decreased（ P 〈0. 05,P 0.01）; the histopathological changes and the myocardial apoptosis were significantly improved. And,the pertreatment effect of BCF 240 mg / kg groups was the most significant（ P 〈0. 01）. Conclusion： BCF could effectively enhance the activity of antioxidant enzymes and free radical scavenging capacity,inhibit the histopathological changes and myocardial apoptosis,suggesting that BCF had dose-dependent protective effects against myocardial ischemia-reperfusion injury in rats.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2015年第1期83-86,共4页
Pharmacology and Clinics of Chinese Materia Medica
关键词
九龙藤总黄酮
心肌
缺血再灌注
自由基
凋亡
Bauhinia championii Benth flavones （九龙藤总黄酮 , BCF）
myocardial
ischemia-reperfusion
free radicals
apoptosis
