摘要
MADS-box家族蛋白是一类重要的调控开花的转录因子，FLC是其家族成员的编码基因之一。FLC在调节开花过程，其功能的发挥受多个途径的调控。本研究利用RT-PCR方法在小黑杨雄花芽中克隆出FLC基因的全长cDNA序列PnFLC。该基因的开放读码框为726bp，编码241个氨基酸残基组成的分子量为27．559kD的蛋白质，蛋白质的等电点为9．37。实时定量荧光PCR结果显示，春化能够使PnFLC在小黑杨根、茎、叶各组织中表达量下调57．9％～84％；启动子GUS染色结果表明，PnFLC启动子在分裂活跃的组织中活性较高。拟南芥的遗传转化结果显示，PnFLC可以调控AtAP1、AtSOC1和AtFT基因的表达，同时延迟拟南芥的开花时间。
MADS-box family protein is an important class of transcription factors regulating flowering. FLC is a member of MADS-box transcription factors,and the FLC gene is controlled by many pathways, and then to regulate the flowering. We cloned a FLC-homolog gene in male flower buds from Populus simonii × Populus nigra. The open reading frame （ORF） of this gene had 726 bp which encoded a protein containing 241 amino acid. The molecular weight of the predicted protein was 27. 559 KD, and the pI was 9.37. By real-time quantitative PCR, vernalization could down-regulate the expression of PnFLC 57.9% - 84% in the root, stem and leaves tissues of poplar. By GUS staining, the promoter of the PnFLC is highly expressed in actively dividing tissues. Furthermore, by transforming Arabopsis, PnFLC down-regulated the expression of AtAP1, AtSOC1 and AtFT, while delayed the flowering in Arabidopsis.
出处
《植物研究》
CAS
CSCD
北大核心
2015年第3期363-369,377,共8页
Bulletin of Botanical Research
基金
国家"863计划"资助项目(2013AA102702)
中央高校基本科研业务费专项资金资助(DL13EA03)
关键词
小黑杨
FLC基因
开花调控
春化
Populus simonii × Populus nigra
FLC gene
flowering
vernalization
作者简介
孙琦（1989－），女，硕士研究生，主要从事林木遗传育种工作。
通信作者：E-mail：liuguanjun2013@nefu．edu．cn
