摘要
从长期生产丁草胺的农药厂排水口土壤中分离得到1株能够降解丁草胺的细菌,将其命名为BTC-3。在以丁草胺为唯一碳源的基础盐培养基中,6 d内可将100 mg/L的丁草胺降解85%以上。经培养特征、生理生化分析和16S rRNA序列分析,将该菌株鉴定为芽孢杆菌属(Bacillus sp.)。菌株BTC-3降解丁草胺的最适温度为30℃,最适p H值为7;当接种量≤3%时,接种量越大,降解率越高;当丁草胺初始浓度≤100 mg/L时,浓度越高,降解效果越好。
A butachlor-degrading bacterial strain named BTC-3 was isolated from a soil sample near the outfall of a pesticide factory, which had produced butachlor for many years. BTC-3 could degrade more than 85% of 100 mg/L butachlor in inorganic salt culture medium with butachlor as sole carbon source within 6 days. Combining the culture, physiological and biochemical character- istics with 16S rRNA sequence analysis, strain BTC-3 was identified as Bacillus sp. The optimal temperature and pH-value for the degradation of butachlor by BTC-3 were 30 ~C and 7, respectively. When the inoculation amount was less than or equal to 3%, the degrading rate increased with the increase in the inoculation amount. When the initial concentration of butaehlor was less than or e- qual to 100 mg/L, the higher the concentration of butachlor, the better the effect of degradation.
出处
《江西农业学报》
CAS
2015年第5期60-64,共5页
Acta Agriculturae Jiangxi
基金
江西省自然科学基金(20114BAB214018)
国家农业公益性行业科研专项(201103007和201203072)资助
关键词
丁草胺
芽孢杆菌属
微生物降解
分离鉴定
降解特性
Butachlor
Bacillus sp.
Biodegradation
Isolation and identification
Degrading characteristics
作者简介
马古平（1981-），男，江苏扬中人，助理研究员，博士，从事环境微生物研究。
