摘要
目的探讨连花清瘟胶囊对脂多糖诱导的急性肺损伤小鼠炎症因子的影响。方法 60只昆明小鼠随机分为正常对照组、模型组、地塞米松组、连花清瘟高(8.04 g生药/kg)、中(4.02 g生药/kg)、低剂量组(2.01 g生药/kg),除正常对照组外,均气管滴注细菌脂多糖(LPS)5 mg/kg进行造模,造模后24 h取血,流式细胞术检测全血肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、白介素8(IL-8)表达;取肺组织,Western blot法检测肺组织磷酸化p38丝裂原活化蛋白激酶(p-p38 MAPK)蛋白表达。结果与模型组相比,连花清瘟高、中剂量组TNF-α、IL-1β、IL-8的表达水平显著降低(P<0.05);连花清瘟高剂量组p-p38 MAPK蛋白表达显著下调(P<0.05)。结论连花清瘟胶囊对LPS诱导的小鼠急性肺损伤有一定的保护作用,其机制可能与调控p38 MAPK通路,抑制炎症因子释放有关。
Objective To explore the effect of Lianhua Qingwen Capsules on the inflammatory factors in mice with acute lung injury（ALI） induced by lipopolysaccharides（LPS）.Methods Sixty KM mice were randomly divided into 6 groups,including control group,model group,dexamethasone group,and high-,middle-and low-dose Lianhua Qingwen groups.All the mice were injected with LPS（5mg/kg） via trachea except control group.After 24 hours,levels of TNF-α,IL-1β and IL-8 in the whole blood were detected by flow cytometry,and p-p38 MAPK protein expression in the lung tissues was also detected by western blot.Results Compared with the model group,expression levels of TNF-α,IL-1β and IL-8 in high-dose and middle-dose Lianhua Qingwen groups decreased significantly（P〈0.05）,and expression level of p-p38 MAPK of high-dose Lianhua Qingwen group was regulated down greatly（P〈0.05）.Conclusion Lianhua Qingwen Capsules show protective effect on acute lung injury induced by LPS,and the mechanisms may be related to the regulation of p38 MAPK passageway and inhibition of the release of inflammatory factors.
出处
《食品与药品》
CAS
2015年第2期96-99,共4页
Food and Drug
基金
河北省中医药管理局科研计划
项目编号2014227
作者简介
张彦芬（1979-），女，工程师，研究方向：中药药理学E—mail：zhangyanfen@yiling．cn
