摘要
目的:研究年龄与性别对二甲基亚硝胺(DMN)中毒所致小鼠急性肝损伤的影响。方法:采用一次性DMN 17.5μl/kg腹腔内注射染毒致4、8、12周龄雌雄性小鼠急性肝损伤,通过肝功能血清生化指标[丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、直接胆红素(DBil)、血清白蛋白(Alb)和总胆固醇(TC)]检测和肝组织HE染色病理学检查,观察不同性别和年龄小鼠急性肝损伤变化特征。结果:DMN染毒48h后致4周龄小鼠血清ALT、AST上升和肝内出血性坏死,以雌性小鼠病变更为明显,部分雌性小鼠出现腹水。8周龄和12周龄小鼠血清ALT、AST上升和肝内出血性坏死更为明显,以雌性小鼠病变极为严重,12周龄小鼠肝呈花斑状,雌性小鼠全部出现腹水并出现死亡,8周龄和12周龄小鼠各有2只雄性小鼠出现腹水。结论:DMN中毒所致小鼠急性肝损伤与年龄和性别密切相关,以雌性、成年小鼠更加明显,表现为肝内出血性坏死和腹水为特征。
Objective：To study the effects of age and gender on dimethylnitrosamine （DMN） -induced acute toxic liver injury in mice. Methods： Male and female mice of 4,8,12 weeks old were intraperitoneally injected with DMN with dosage of 17.5μl /kg once a time to in-duce acute liver injury, after 48 hours serum biochemical parameters of liver function [including albumin（Alb）, alanine aminotransferase （ALT） ,aspartate aminotransferase （AST）, direct bilirubin （DBil）,total cholesterol（TC）] and HE staining of liver tissue were detected to observe the characteristic changes of acute liver injury with different age and gender.Results：Forty-eight hours after the intraperitoneal injec-tion of DMN with dosage of 20mg/kg once a time caused increase of serum ALT, AST and DBil, decrease of serum of Alb and TC,hemor-rhagic necrosis of the liver in mice of 4 weeks old.Those changes were significantly evident in the female mice, some female mice had asci-tes.With the increase of age, the above changes became more evident, all female aged mice of 8 and 12 weeks old had serious ascites, and some male aged mice had ascites, deaths only occurred in female aged mice of 8 and 12 weeks old.Conclusion：The acute liver injury in mice induced by DMN is closely related with age and gender, the injury is more pronounced in female aged mice,which manifestations are hemor-rhagic liver necrosis and ascites.
出处
《中西医结合肝病杂志》
CAS
2015年第2期92-94,I0002,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
上海中医药大学实验技术人才队伍建设资助计划(No.上中医〔2012〕人字18号)
国家中医药管理局中医肝胆病重点学科(NO:2010sh)
上海高校创新团队建设项目
上海市教委创新团队(第一期)
国家教育部博士点专项基金(20123107110003)
上海市科委临床重点项目(11411951600)
上海市科委科技支撑项目(No.13401902900)
上海市中医药事业发展三年行动计划项目(No.ZYSNXD-CC-ZDYJ015/048)
上海中医药大学预算内项目(2013JW40)
关键词
二甲基亚硝胺
小鼠
年龄
性别
急性肝损伤
出血性坏死
腹水
dimethylnitrosamine
mice
age
gender
acute liver injury
hemorrhagic liver necrosis
ascites
作者简介
通讯作者，E—mail：jinshugen@hotmail．com
