摘要
应用虎红平板凝集试验(Rose-Bengal Plate Agglutination Test,RBPT)、衣原体、弓形虫和绵羊肺炎支原体间接血凝试验(Indirect Hemagglutination Assay,IHA),对2013年采自青海省同德县部分乡镇的201份绵羊血清样品进行了布鲁氏菌、衣原体、弓形虫和绵羊肺炎支原体的血清学检测。结果:未检出布氏杆菌阳性血清;分别检出衣原体、弓形虫和绵羊肺炎支原体阳性血清2份、4份和7份,其阳性率分别为1.00%、1.99%和3.48%。此外,对于抗体阳性的血清样品采用PCR方法进行了抗原检测,结果表明,青海省同德县地区绵羊中存在衣原体、弓形虫和绵羊肺炎支原体的感染。
To investigate the existence prevalence of Brucellosis, Chlamydiosis, Toxoplasmosis and Mycoplasma Ovipneumoniae of 201 sheep serum samples in Tongde County of Qinghai Province, Rose-Bengal Plate Agglutination Test（RBPT）, Chlamydia, Toxoplasma, and Mycoplasma Ovipneumoniae Indirect Hemagglutination Assay（IHA）were used to detect antibody of these disease. The results showed that2 of the 201 samples were Chlamydiosis positive, 4 samples were Toxoplasmosis positive, 7 samples were Mycoplasma Ovipneumoniae positive and the positive rates were 1.00 percent, 1.99 percent and 3.48 percent, respectively. Brucellosis were not detected. In addition, the antigen were detected by PCR method using the sample of serum antibody positive. In conclusion, the sheep in Tongde County were infected by Chlamydia, Toxoplasma and Mycoplasma Ovipneumoniae.
出处
《青海畜牧兽医杂志》
2015年第2期8-9,共2页
Chinese Qinghai Journal of Animal and Veterinary Sciences
基金
现代农业产业技术体系建设专项经费资助(CARS-40-08B)
青海省现代农牧业产业科技创新平台-羊产业科技创新平台
关键词
绵羊
布鲁氏菌
衣原体
弓形虫
肺炎支原体
检测
Sheep
Brucella
Chlamydia
Toxoplasma
Mycoplasma Ovipneumoniae
Detection
作者简介
通讯作者：马利青，研究员，主要从事动物疫病诊断和防治研究，E—mail：maliq67@hotmail．com.
