摘要
目的 :观察人源抗Trop-2 Fab对宫颈癌细胞生物学特性的影响。方法 :流式细胞术和免疫荧光检测宫颈癌Hela细胞表面Trop-2蛋白的表达;划痕实验和Transwell检测人源抗Trop-2 Fab对宫颈癌细胞迁移能力的影响;流式细胞术检测人源抗Trop-2 Fab对宫颈癌细胞凋亡的诱导作用;CCK-8(cell counting kit-8)检测人源抗Trop-2 Fab对宫颈癌细胞增殖的影响。结果:Hela细胞表面有Trop-2蛋白表达,人源抗Trop-2 Fab能够抑制Hela细胞的增殖,有效降低Hela细胞的迁移和侵袭能力,同时能够诱导Hela细胞凋亡。结论:人源抗Trop-2 Fab能明显降低宫颈癌细胞的恶性生物学行为,Trop-2可作为宫颈癌治疗的潜在靶点。
Objective：To investigate effects of human anti-Trop-2 Fab antibody on biological characteristics of cervical cancer cells. Methods：The expression of Trop-2 protein from cervical cancer cells was assessed by flow cytometry（FCM）and immunofluorescence. The effect of human anti-Trop-2 Fab antibody on migration in human cervical cells was examined by wound healing assay and transwell test. Cell apoptosis induced by Trop-2 Fab was analyzed by flow cytometry. Human anti-Trop-2 Fab antibody influence on cervical cell proliferation was detected by cell counting kit-8（CCK8）assay. Results：Trop-2 protein was confirmed to express on human cervical cells. Human anti-Trop-2 Fab antibody inhibited human cervical cancer cell growth, migration and invasion, at the same time induced cervical cancer cell apoptosis. Conclusion：Human anti-Troo-2 Fab antibody can significantly inhibit the malignant biological behavior of cervical cancer cells. This fusion antibody may play a role in tumor targeting therapy, and Trop-2 may be a potential therapeutic target for cervical cancer.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第3期320-325,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京市医学科技发展项目(ZKX12025)
南京市科学技术委员会第八批科技发展项目(201208025)
江苏省妇幼保健科研项目(F201211)
关键词
人源抗Trop2抗体
宫颈癌
生物学特性
human anti-Trop2 Fab antibody
cervical cancer
biological characteristics
作者简介
通信作者（Corresponding author），E—mail：thua8@163．com；zhujin1968@njmu．edu．cn
