摘要
脱落酸非敏感型转录因子ABI4、生长素运输载体蛋白PIN1分别通过调控脱落酸和生长素的运输而影响植物不定根的发育与生长。以经生根粉诱导处理和未经诱导处理的桑树绿枝插穗为材料,用实时荧光定量PCR、Western blot技术检测插穗生根发育过程中基部皮层ABI4和PIN1基因的转录与蛋白质表达水平变化。当插穗出现不定根时,ABI4的转录与表达水平急剧下调,PIN1的转录水平急剧上调,而后在新生根伸长期二者均有所回复;生根剂诱导处理插穗后加大了其基部皮层ABI4的转录与表达下调幅度和PIN1的转录上调幅度,且PIN1在新生根伸长期持续高表达。ABI4的转录水平与不同桑品种生根率之间均呈显著的负相关关系(γ=-0.915),与生根数量没有相关性;而PIN1的转录水平与生根率和生根数量均呈显著的正相关关系(γ=0.880,γ=0.982)。农桑14号和强桑1号等是绿枝生根率高的桑品种,其插穗不定根出现时基部皮层ABI4的转录、表达下调幅度和PIN1的转录上调幅度大于大10等生根率低的桑品种,提示易生根品种的插穗在不定根出现时期ABI4的低水平和PIN1的高表达会更利于插穗根原基的形成、分化及新生根的生长。
Abscisic acid insensitive 4（ ABI4） and peptidyl-prolyl cis-trans isomerase（ PIN1） in plants play importantroles in adventitious root development and growth by regulating abscisic acid and auxin transport,respectively. Using mulberry softwood cuttings before and after induction with rooting powder,the transcription and protein expression levels of ABI4 and PIN1 genes in basal cortex during the rooting process of cuttings were measured by real-time fluorescent quantitative PCR and Western blotting. The results showed that the transcription and protein expression levels of ABI4 were dramatic-ally down-regulated,while the expression levels of PIN1 were significantly up-regulated at adventitious root projecting stage. The expression levels of both genes were slightly recovered during the new root elongation stage. In treatment group with rooting agents,down-regulation amplitude of the transcription and protein expression levels of ABI4 and upregulation amplitude of the transcriptional levels of PIN1 were much higher than those of control group,and PIN1 was expressed continuously at high level during the new root elongation stage. The transcription levels of ABI4 were significantly negatively correlated with the rooting rates of all mulberry varieties（ γ =- 0. 915）,but was not correlated with the rooting amount. However,the transcriptional levels of PIN1 were significantly positively correlated with both rooting rates and rooting amounts（ γ = 0. 880,γ = 0. 982）. Furthermore,the down-regulation amplitude of the transcription and protein expression levels of ABI4 and up-regulation amplitude of the transcriptional levels of PIN1 in mulberry varieties with high rooting rate such as Nongsang 14 and Qiangsang 1 were higher than those in mulberry varieties with low rooting rate such as Da 10,indicating that the low protein expression level of ABI4 and the high level of PIN1 gene expression in cuttings of the easy-rooting mulberry varieties are beneficial to formation and differentiation of root primordia and to growth of new roots.
出处
《蚕业科学》
CAS
CSCD
北大核心
2015年第2期204-210,共7页
ACTA SERICOLOGICA SINICA
基金
国家农业科技成果转化基金项目(No.2012C22086)
浙江省农业科学院创新提升和国际合作项目(No.2014CX005
2011R06Y01E02
2012R06A00D01)
浙江省绍兴330英才项目(No.CXYC2012005)
作者简介
裘晓云（1966-），女，副研究员。Tel：0571—86404298，E—mail：guoxinshen@gmail．com
通信作者信息：沈国新，研究员，硕士生导师。Tel：0571-86404298，E-mail：guoxin．shen@ttu．edu
