摘要
目的:探讨A型肉毒毒素(BTXA)对增生性瘢痕组织成纤维细胞中TGF-β1和cyclinD1表达的影响。方法:组织块法培养增生性瘢痕组织及正常皮肤组织的成纤维细胞,用不同浓度的BTXA作用于体外培养的人增生性瘢痕成纤维细胞,检测MTT值;利用RT-PCR分析用药前后TGF-β1和cyclinD1m RNA表达的变化及其他们的相关性。结果:BTXA在体外能明显抑制增生性瘢痕成纤维细胞的增殖及TGF-β1和cyclinD1m RNA的表达。结论:BTXA对增生性瘢痕组织中成纤维细胞的增殖有明显抑制作用,其作用机制与BTXA能抑制TGF-β1和cyclinD1m RNA的表达有关;同时在抑制成纤维细胞的过程中,TGF-β1和cyclinD1m RNA的表达并无相关性。
Objective To investigate the effect of botulinum toxin A （BTXA）on the expression of TGF-β1 and cyclinD1 of hypertrophic scar fibroblast. Methods After treated with different concertrations of BTXA,inhibition rate.The expression and correlation of TGF- β1 of cyclinD1of hypertrophic scar fibroblasts were detected by MTT assay and RT-PCR respectively. Results BTXA can significantly inhibit the proliferation of hypertrophic scar fibroblast,and the expression of TGF-β1 and cyclinD1 could be inhibited significantly. Conclusion The mechanism of BTXA on inhibiting fibroblast of hypertrophic scar may be related with regulation of the expression of TGF-β1 of cyclinD1,and there was no correlation between the expression of TGF-1 and cyclinD1mRNA on inhibiting fibroblast of hypertrophic scar.
出处
《中国美容医学》
CAS
2015年第6期37-41,共5页
Chinese Journal of Aesthetic Medicine
关键词
A型肉毒毒素
增生性瘢痕
botulinum toxin A（BTXA）
hypertrophic scar
TGF-β1
cyclinD1
作者简介
通信作者：王连英，主任医师，河北省秦皇岛市，秦皇岛市第一医院烧伤整形外科，066000
