摘要
目的：建立拉曼光谱法快速测定盐酸异丙嗪注射液的含量。方法：采用智能激光拉曼光谱仪扫描样品,激发波长780nm,激光强度100 MW,光圈25μm,光栅400 gr·mm-1,分辨率2.0cm-1,曝光时间50 s,曝光次数2次,扫描范围100~3 200cm-1。结果：盐酸异丙嗪在20~200 mg·ml-1范围内与拉曼定量峰面积呈良好的线性关系（r=0.998 2）,平均加标回收率为98.13%,RSD为1.05%（n=9）。结论：本方法快速、简单、可靠,可用于盐酸异丙嗪注射液的含量测定。
OBJECTIVE To establish a rapid raman spectroscopy method for content determination of promethazine hydrochloridne injection.METHODS Smart laser raman spectrometer was used for determination.The excitation wavelength was780 nm,laser power 100 MW,aperture 25μm,grating400 gr·mm-1,resolution2.0cm-1,exposure time 50 s,exposure times2,and the scanning range 100- 3 200 cm-1.RESULTS The calibration curve was linear over the range of 20-200 mg·ml-1（r=0.998 2）,and the average recovery was 98.13% with RSD of 1.05%（n=9）.CONCLUSION The method is convenient and rapid.It is able to be used in the rapid determination of injection.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2015年第7期612-615,共4页
Chinese Journal of Hospital Pharmacy
基金
福建省教育厅科技类项目(编号:JA13451)
关键词
拉曼光谱
盐酸异丙嗪
注射液
快速测定
Raman spectra
promethazine hydrochlorine
injection
rapid determination
作者简介
赖源发，男，硕士，主管药师，研究方向：药品质量研究与控制，电话：0592—6276259，E-mail：laiyuanfa@gmail．com.
