摘要
目的探讨STAT3在人肝内胆管癌细胞系增殖与凋亡中的作用及其机制。方法采用Western blot法和Real-time PCR法检测人肝内胆管癌细胞系RBE、9810(肝内组)、人肝外胆管癌细胞系QBC939、SSP-25、KMBC(肝外组)STAT3蛋白和STAT3 mRNA表达水平;将肝内组细胞分为干扰组和对照1组,细胞内分别瞬时转染干扰STAT3表达的腺病毒和对照腺病毒各5μL,干预48 h;另将肝内组细胞分为过表达组和对照2组,细胞内分别瞬时转染过表达STAT3的腺病毒及对照腺病毒5μL,干预48 h。分别采用CCK8法检测各组细胞增殖情况,采用PIAnnexin V法检测细胞的凋亡情况。结果肝内组STAT3蛋白及STAT3 mRNA表达水平均高于肝外组,P均<0.05。干扰组RBE、9810细胞增殖能力均低于对照1组,细胞凋亡率均明显高于对照1组,P均<0.05。过表达组RBE、9810细胞增殖能力高于对照2组,细胞凋亡率明显低于对照2组,P均<0.05。结论 STAT3在促进肝内胆管癌细胞增殖、抵抗细胞凋亡中发挥重要作用。
Objective To investigate the effect and mechanism of signal transducer and activator of transcription 3（ STAT3） on the proliferation and apoptosis of human intrahepatic cholangiocarcinoma cells. Method We detected the STAT3 protein and mRNA levels in human cholangiocarcinoma cells by Western blotting and real-time PCR. We divided the cells of the intrahepatic group into the interference group and the control group 1,and cells were treated with the adenovirus 5 μL for 48 h to knockdown the expression of STAT3. Furthermore,We divided the cells of the intrahepatic group into the overexpression group and the control group 2,and the cells were treated with the adenovirus 5 μL for 48 h to over-express the expression of STAT3.We used the CCK8 to detect the cell proliferation of all group. We used the PI-Annexin V to detect the cell apoptosis of all group.Results The mRNA and protein levels of STAT3 in the intrahepatic group were higher than those in the extrahepatic group（ all P〈0. 05）. The RBE and 9810 cell proliferation abilities of the interference group were lower than those of the control group 1（ all P〈0. 05）; and the RBE and 9810 cell apoptosis rates of the interference group were higher than those of the control group 1（ all P〈0. 05）. The RBE and 9810 cell proliferation abilities of the overexpression group were higher than those of the control group2（ all P〈0. 05）; and the RBE and 9810 cell apoptosis rates of the overexpression group were lower than those of the control group 2（ all P〈0. 05）. Conclusion STAT3 plays an important role in promoting intrahepatic cholangiocarcinoma cell proliferation and anti-apoptosis.
出处
《山东医药》
CAS
北大核心
2015年第14期5-7,共3页
Shandong Medical Journal
基金
国家自然科学基金资助项目(81372188)
作者简介
刘闪闪（1990-），女，硕士，研究方向为肿瘤的发生发展。E—mail：shanshanliu90@163．com
通信作者简介：高建新（1956-），男，教授，博士生导师，研究方向为肿瘤的发生发展。E-mail：15618820486@163．com
