摘要
目的：构建多糖-蛋白质细胞外基质（ECM）纳米复合材料,探究果胶比例复合材料生物学特性的影响.方法：以壳聚糖/明胶/果胶为基材,通过接枝耦联反应,引入活性基团,经纳米羟基磷灰石的原位生成反应,制作具有纳米微环境的多糖-蛋白质ECM复合材料.根据果胶的不同比重构建5组（10％、30％、50％、70％、0％）复合材料,分别与骨髓间充质干细胞（MSCs）复合培养.通过四甲基偶氮唑盐（MTT）法检测材料的细胞黏附活性和增殖活性,优选复合材料;通过扫描电镜和F-DA荧光染色法评价优选复合材料的生物相容性.结果：MTT显示30％果胶组材料具有最好的黏附性和最高的增殖率.扫描电镜和F-DA荧光染色结果显示该组材料复合培养的细胞生长状态良好,形态饱满,黏附紧密.30％果胶组材料蕴含丰富的生物信息.结论：30％果胶比重的多糖-蛋白质ECM纳米复合材料细胞毒性小,生物相容性好,是一种极具前景的骨组织工程材料.
Objective： To construct pectin-based polysaccharide-protein extracellular matrix （ECM） nano composition and study its role in the composition Methods： As substrate, given the chitosan/gelatin/pectin biological intelligence activity by grafting coupling reaction, further by irrsitu reaction of nano hydroxyapatite, structured nano microenvironment of the polysaccharide protein ECM composite material. According to different proportions of pectin, composite materials were divided into five groups （10 %, 30%, 50%, 70%, 0%）. Bone marrow mesenchymal stem cells co-cultured with the five groups as the experimental group. The composition was optimized based cell adhesion and proliferation activity by MTT and biocompatibility was evaluated by scanning electron microscopy and F-DA fluorescence staining. Results： The results of MTT assay showed that the one with 30% pectin had the best adhesion and the highest proliferation rate. Scanning electron microscopy and F-DA staining results showed that the cells grew well and adhered tight in this group. This biological information contained material had good bioeompatibility. Conclusion： The composite material with 30 % pectin is a promising bone tissue engineering bioraaterial with its low cytotoxicity and high biocomDatibilitv.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2015年第1期27-30,共4页
Chinese Journal of Anatomy
基金
国家自然科学基金(81101448/H1511)
河北省自然科学基金(C2011401006,H2012401017)
河北省引进留学人员资助项目(C201400560)
关键词
果胶
细胞外基质
间充质干细胞
生物相容性
pectim extracellular matrix
mesenchymal stem cells
biocompatibility
作者简介
E-mail：jiyaming1118@hotmail．com
通讯作者，E-mail：xsunl@hotmail．com
