摘要
为筛选新的高产纤维素酶真菌,从长期堆放生物质废弃物土壤中分离到1株产纤维素酶真菌。经形态特征观察、ITS分析,初步鉴定为曲霉属,暂定名为Aspergillus cel403。通过单因素试验研究了不同碳源、氮源浓度和培养基初始p H值对该菌在液体发酵中产纤维素酶的影响,在此基础上运用响应面法分析其最佳发酵条件。结果表明,Aspergillus cel403产纤维素酶最佳条件:培养基组分为CMC-Na 15.60 g、KH2PO41.00 g、Mg SO4·7H2O 0.50g、Na Cl 0.10 g、Na NO32.50 g、Fe Cl31 mg、Ca Cl20.10 g、酵母膏1.09 g,H2O 1 000 ml,p H7.1;30℃、140 r/min摇床培养6 d。该条件下发酵产纤维素酶活性为89.66 U/ml,比未经优化发酵条件对照提高了15.02%。可见,Aspergillus cel403在纤维素降解利用方面具备进一步开发潜力。
To screen and characterize novel fungi with powerful cellulose degradation capability,a cellulose-decomposing fungus was isolated from soil rich in biomass wastes. Based on morphology and ITS sequence analysis,the fungus was initially classified into the genus of Aspergillus and temporarily named as strain cel403. The influence of carbon and nitrogen sources concentrations,and initial p H were studied through single factor experiment on the cellulase production. The response surface method was used to analyse its optimum fermentation condition for cel403,which was shown as follows：the medium was comprised of CMC-Na 15. 60 g,KH2PO41. 00 g,Mg SO4·7H2O 0. 50 g,Na Cl 0. 10 g,Na NO32. 50 g,Fe Cl31 mg,Ca Cl20. 10 g,yeast extract 1. 09 g,H2 O 1 000 ml,with p H value at 7. 1,and cel403 was cultured at 30 ℃for 6 d. The cellulose production under above conditions was 89. 66 IU / ml, increased by 15. 02% compared to control. Aspergillus cel403 is a candidate strain for the degradation of cellulose.
出处
《江苏农业学报》
CSCD
北大核心
2015年第1期186-191,共6页
Jiangsu Journal of Agricultural Sciences
基金
广东省2012科技计划项目(2012B020420003)
揭阳职业技术学院2013科研重点课题(JYCKZ1306)
揭阳职业技术学院2014~2016年"创新强校"自主能力提升项目
关键词
纤维素降解菌
筛选
鉴定
ITS
产酶条件
响应面法
cellulose-decomposing fungus
screening
identification
ITS
enzyme-producing condition
response surface method
作者简介
杨培新（1971-），男，广东揭阳人，硕士，教授，主要从事农业生物技术相关教学与研究工作。
通讯作者：郑锐东，（E-mail）superzrd@163．com
