摘要
为了解维拉帕米对恩诺沙星在脊尾白虾体内代谢及消除的影响,在水温(18.1±0.5)℃条件下,对照组每天以10mg/kg体重剂量连续7天投喂脊尾白虾恩诺沙星,维拉帕米组在投喂恩诺沙星的同时以4 mg/L剂量药浴脊尾白虾维拉帕米,比较了维拉帕米组和对照组中恩诺沙星及其代谢物环丙沙星的浓度变化与消除动力学统计矩参数。结果显示,肝胰腺、肌肉、和鳃3种组织中的恩诺沙星浓度均随时间呈规律性递减,维拉帕米组3种组织中的恩诺沙星浓度、AUC和t1/2β均高于对照组(P<0.05),而维拉帕米组各组织中的环丙沙星的浓度和AUC却显著低于对照组(P<0.05);维拉帕米组3种组织中恩诺沙星和环丙沙星的β、CL均显著小于对照组(P<0.05)。结果表明,维拉帕米抑制了脊尾白虾体内恩诺沙星的代谢及消除速率。
The purpose of this study was to investigate the effect of verapamil on metabolism and elimination of enrofloxacin in ridgetail white prawn, Exopalaemon carinicauda. The shrimps were randomly divided into two groups with different experimental diets. The first group was fed enrofloxacin diet at a dosage of 10 mg/kg · d for seven days and served as control. The second group was fed enrofloxacin diet at a dosage of 10 mg/kg·d for seven days, and simuhaneously bathed with verapamil at 4 mg/L for seven days, and as verapamil group. The drug concentrations and elimination parameters were compared. The results showed that the enrofloxcin concentration decreased with the days in hepatopancreas, muscle and gill, and the concentrations of enrofloxacin in tissues were significantly higher in verapamil group than those in control group （ P 〈 0.05 ）, however, the concentrations of enrofloxacin metabolite ciprofloxacin in tissues were significantly lower in verapamil group than those of control group （ P 〈 0.05 ）. The elimination rate constant （13） and clearance rate （CL） of enrofloxacin and ciprofloxacin were significantly lower in verapamil group than those in control group （ P 〈 0.05 ）. The results showed that verapamil inhibited the metabolism and elimination of enrofloxacin in E. carinicauda. Especially, P-glycoprotein might be involved in the elimination of enrofloxacin and ciprofloxacin in E. carinicauda.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2015年第2期219-226,共8页
Journal of Shanghai Ocean University
基金
国家虾产业技术体系(CARS-47)
公益性行业(农业)科研专项经费项目(201103034)
河南师范大学博士科研启动经费项目(QD13054)
关键词
脊尾白虾
维拉帕米
P-糖蛋白
恩诺沙星
环丙沙星
Exopalaemon carinicauda
verapamil
P-glycoprotein
enrofloxacin
ciprofloxacin
作者简介
梁俊平（1981-），男，讲师，博士，研究方向为水产动物营养与安全。E-mail：Liangjunpinglover@163．com
通信作者：李健，E-mail：Lijian@ysfri．ac．cn
